In Vitro Cultivation of Cells Isolated from Bone Marrow
6:18
Collecting Synovial Fluid and Cultivating Isolated Cells
8:19
Results: Bone Marrow- and Synovial Fluid-derived MSC Morphology and Cell Surface Marker Expression
9:23
Conclusion
Transcrição
The overall goal of this procedure is the non-invasive collection of synovial fluid and bone marrow from minipigs to collect mesenchymal stem cells to be used in regeneration and immunomodulation studies. This method can help answer key questions
Faça login ou inicie seu teste gratuito para acessar este conteúdo
A protocol establishing mesenchymal stem cells (MSCs) isolated from the synovial fluid and bone marrow of minipigs and non-invasively collected using syringe aspiration is presented. Cellular phenotyping was performed using flow cytometry after cell isolation and in vitro cultivation of bone marrow and synovial fluids derived from MSCs.