Primary Antibodies and Proximity Ligation Assay Probes
6:09
Amplification and Imaging Preparation
7:48
Results: In Situ Proximity Ligation Assay in HuH7 Cells
8:52
Conclusion
TRANSCRIÇÃO
The overall goal of this experiment is to visualize and quantify endoplasmic reticulum mitochondria interactions using an in situ proximity ligation assay in fixed cells.This method can help to answer key questions in the biomedical field to bette
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Here, we describe a procedure to visualize and quantify with high sensitivity the endogenous interactions between the endoplasmic reticulum and mitochondria in fixed cells. The protocol features an optimized in situ proximity ligation assay targeting the inositol 1,4,5-triphosphate receptor/glucose-regulated protein 75/voltage-dependent anion channel/cyclophilin D complex at the mitochondria-associated membrane interface.