Results: Evaluation of Elution Patterns with Sequential Salt Extraction
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Conclusion
TRANSCRIÇÃO
The overall goal of the Sequential Salt Extraction procedure is to characterize protein binding profiles to bulk chromatin and to valuate how the binding is altered when the protein, or its environment, is manipulated. This method measures the rel
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Sequential salt extraction of chromatin bound proteins is a useful tool for determining the binding properties of large protein complexes. This method can be employed to evaluate the role of individual subunits or domains in the overall affinity of a protein complex to bulk chromatin.