Metaphase I (MI) or Metaphase II (MII) Oocyte Collection
5:17
Oocyte Denuding and Zona Pellucida Removal
7:05
MI and MII Oocyte Chromatin Spreads
8:08
Results: Crossover Formation in C57B/6J Oocytes
9:52
Conclusion
Transcrição
The overall goal of this chromatin spread preparation is to chronologically visualize the dynamic localization patterns of chromatin-bound proteins and chromosome morphology of oocytes throughout mammalian oogenesis. This method can help answer ke
Faça login ou inicie seu teste gratuito para acessar este conteúdo
Oogenesis in mammals is known to be error-prone, particularly due to chromosome missegregation. This manuscript describes chromatin spread preparation methods for mouse prophase, metaphase I and II-staged oocytes. These fundamental techniques allow for the study of chromatin-bound proteins and chromosome morphology throughout mammalian oogenesis.