Diagnosing Neoplastic Phenotypes from Confocal Image
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Results: Tumors Induction from IgI-RNAi Expression in the Wing Disc
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Conclusion
Transcrição
The overall goal of this procedure, is to generate tumerogenic mosaic clones in developing Drosophila imaginal discs, and then classify the clonal lesions into various stages of tumor development. This method can help answer a key question in canc
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Mosaic clone analysis in Drosophila imaginal disc epithelia is a powerful model system to study the genetic and cellular mechanisms of tumorigenesis. Here we describe a protocol to induce tumors in Drosophila wing imaginal discs using the GAL4-UAS system, and introduce a diagnosis method to classify the tumor phenotypes.