Obtaining Cancer Stem Cell Spheres from Gynecological and Breast Cancer Tumors

DOI :

10.3791/60022-v

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March 1st, 2020

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9,602 Views

Mafalda Laranjo^1,2,3,4*, Maria João Carvalho^1,2,3,4,5,6*, Beatriz Serambeque^1,2,3, André Alves^2,7, Carlos Miguel Marto^1,2,3,4,8, Isabel Silva⁹, Artur Paiva^3,9,10, Maria Filomena Botelho^1,2,3,4,8

¹Institute of Biophysics, Faculty of Medicine, University of Coimbra, ²Coimbra Institute for Clinical and Biomedical Research (iCBR), area of Environment Genetics and Oncobiology (CIMAGO), Faculty of Medicine, University of Coimbra, ³CNC.IBILI/Center for Innovative Biomedicine and Biotechnology (CIBB), University of Coimbra, ⁴Clinical Academic Center of Coimbra (CACC), ⁵Universitary Clinic of Gynecology, Faculty of Medicine, University of Coimbra, ⁶Gynecology A Service, Coimbra Hospital and Universitary Center, ⁷Institute of Pharmacology & Experimental Therapeutics, Faculty of Medicine, University of Coimbra, ⁸Institute of Experimental Pathology, Faculty of Medicine, University of Coimbra, ⁹Cytometry Operational Management Unit, Clinical Pathology Service, Coimbra Hospital and Universitary Center, ¹⁰Polytechnic Institute of Coimbra, ESTESC-Coimbra Health School, Laboratory Biomedical Sciences

Capítulos

The aim of this methodology is to identify cancer stem cells (CSC) in cancer cell lines and primary human tumor samples with the sphere-forming protocol, in a robust manner, using functional assays and phenotypic characterization with flow cytometry and Western blot.

Tags

Keywords Cancer Stem Cells

Cancer Stem Cell Spheres

Sphere forming Assay

PolyHEMA

Non adherent Suspension Culture

Epidermal Growth Factor

Basic Fibroblast Growth Factor

Sphere forming Capacity

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