Determining Relative Cell-surface Protein Expression in Astrocytes by Biotinylation
4:40
Determining Internalization Rate of Cell-surface Proteins in Astrocytes by Biotinylation
8:52
Results: AQP4 Expression at the Cell-surface in the Presence of Laminin and AQP4 Internalization in Astrocytes
10:15
Conclusion
TRANSCRIÇÃO
In this video, two methods will be presented, cell-surface biotinylation, and pulse-chase biotinylation. In the following demonstration, we will be using astrocytes. These are the most abundant glial cell types in the brain, and they happen to exp
Sign in or start your free trial to access this content
Two biotinylation-based methods, designed for determining the cell-surface expression and endocytic rate of proteins expressed at the plasma membrane, are presented in this report.