Targeting Cysteine Thiols for in Vitro Site-specific Glycosylation of Recombinant Proteins

1:09

Polymerase Chain Reaction (PCR)-mediated Site-directed Mutagenesis

3:44

Uniform ¹⁵N-labeled Protein Expression in BL21 ΔE3 E. coli

5:24

Purification of Recombinant Protein from E. coli

7:50

Chemical Attachment of Glucose-5-MTS to Protein by Dialysis

8:48

Results: Site-selective Cys Mutation Followed by In Vitro Glycosylation in STIM1 EF-SAM Protein

10:11

Conclusion