6.5K Views
•
11:25 min
•
October 4th, 2017
DOI :
October 4th, 2017
•Chapters in this video
0:05
Title
1:09
Polymerase Chain Reaction (PCR)-mediated Site-directed Mutagenesis
3:44
Uniform 15N-labeled Protein Expression in BL21 ΔE3 E. coli
5:24
Purification of Recombinant Protein from E. coli
7:50
Chemical Attachment of Glucose-5-MTS to Protein by Dialysis
8:48
Results: Site-selective Cys Mutation Followed by In Vitro Glycosylation in STIM1 EF-SAM Protein
10:11
Conclusion
Related Videos
Generation of Recombinant Arenavirus for Vaccine Development in FDA-Approved Vero Cells
16.8K Views
Expression of Functional Recombinant Hemagglutinin and Neuraminidase Proteins from the Novel H7N9 Influenza Virus Using the Baculovirus Expression System
23.9K Views
Monitoring the Assembly of a Secreted Bacterial Virulence Factor Using Site-specific Crosslinking
6.1K Views
Intranasal Administration of Recombinant Influenza Vaccines in Chimeric Mouse Models to Study Mucosal Immunity
12.5K Views
Evaluation of the Efficacy And Toxicity of RNAs Targeting HIV-1 Production for Use in Gene or Drug Therapy
7.8K Views
Murine Lymphocyte Labeling by 64Cu-Antibody Receptor Targeting for In Vivo Cell Trafficking by PET/CT
8.7K Views
System for Efficacy and Cytotoxicity Screening of Inhibitors Targeting Intracellular Mycobacterium tuberculosis
8.5K Views
In Vitro Methods for Comparing Target Binding and CDC Induction Between Therapeutic Antibodies: Applications in Biosimilarity Analysis
17.6K Views
Separation of Rat Epidermis and Dermis with Thermolysin to Detect Site-Specific Inflammatory mRNA and Protein
5.2K Views
Highly Efficient Transfection of Primary Macrophages with In Vitro Transcribed mRNA
22.1K Views
Copyright © 2025 MyJoVE Corporation. All rights reserved