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JoVE Journal

Medicine

This content is Open Access.

Studying the Hypothalamic Insulin Signal to Peripheral Glucose Intolerance with a Continuous Drug Infusion System into the Mouse Brain

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08:32 min

January 4th, 2018

January 4th, 2018

10,086 Views

0:05

Title

1:21

Preparation of Micro-osmotic Pump Infusion Systems

2:41

Intracerebral Ventricular Surgery - Implantation of the Micro-osmotic Pump

4:15

Oral Glucose Tolerance Test (OGTT) and Insulin Tolerance Test (ITT)

6:04

Results: Glucose Metabolism and Insulin Signal Activation

7:27

Conclusion

This protocol studies the role of chemokine (C-C motif) ligand 5 (CCL5) in the hypothalamus by delivering an antagonist, MetCCL5, into the mouse brain using a micro-osmotic pump brain infusion system. This transient inhibition of CCL5 activity interrupted hypothalamic insulin signaling, leading to glucose intolerance and peripheral systemic insulin sensitivity.

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