Preparing Molds and Mounting Agarose and Embedding Embryos
3:31
Photoconversion
5:12
Unmounting Photoconverted Embryos
6:20
Imaging Photoconverted Cells at Later Embryonic Stages
8:05
Results: Photoconversion of Kaede Fluorescent Protein in Zebrafish Embryonic Endocardial Cells
9:01
Conclusion
Transcrição
The overall goal of this procedure is to follow endocardial cells during atrioventricular canal and heart valve formation. This method can help to address key question in the field of cardiac development, such as to understand how endocardial cell
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This protocol describes a method for the photoconversion of Kaede fluorescent protein in endocardial cells of the living zebrafish embryo that enables the tracking of endocardial cells during atrioventricular canal and atrioventricular heart valve development.