Using In Vitro Fluorescence Resonance Energy Transfer to Study the Dynamics Of Protein Complexes at a Millisecond Time Scale

0:33

Designing the FRET Assay

1:12

Preparation of Cul1^AMC•Rbx1, the FRET Donor Protein

4:42

Preparation of ^FlAsHCand1, the FRET Acceptor Protein

5:18

Testing and Confirmation of the FRET Assay

6:08

Measuring the Association Rate Constant

7:37

Measuring the Dissociation Rate Constant of Cul1•Cand1 in the Presence of Skp•F-box Protein

8:33

Results: Dynamics Of Protein Complexes

10:30

Conclusion