October 27th, 2020
•This protocol provides an efficient collagenase digestion method for isolation of viable adipocytes and stromal vascular fraction-SVF cells from human visceral fat in a single process, including methodology to obtain high-quality RNA from adipocytes and phenotypification of SVF-macrophages through staining of multiple membrane-bound markers for analysis by flow cytometry.
Tags
Vídeos Relacionados
Isolation of Human Islets from Partially Pancreatectomized Patients
Experimental Generation of Carcinoma-Associated Fibroblasts (CAFs) from Human Mammary Fibroblasts
Isolation of Cardiomyocyte Nuclei from Post-mortem Tissue
Encapsulation Thermogenic Preadipocytes for Transplantation into Adipose Tissue Depots
Isolation and Cellular Phenotyping of Mesenchymal Stem Cells Derived from Synovial Fluid and Bone Marrow of Minipigs
Stromal Vascular Fraction-enriched Fat Grafting for the Treatment of Symptomatic End-neuromata
Assessment of Human Adipose Tissue Microvascular Function Using Videomicroscopy
Isolation of Atrial Cardiomyocytes from a Rat Model of Metabolic Syndrome-related Heart Failure with Preserved Ejection Fraction
Clinical Protocol of Producing Adipose Tissue-Derived Stromal Vascular Fraction for Potential Cartilage Regeneration
Isolation of Adipose Derived Regenerative Cells for the Treatment of Erectile Dysfunction Following Radical Prostatectomy
SOBRE A JoVE
Copyright © 2024 MyJoVE Corporation. Todos os direitos reservados