December 1st, 2020
•Neutron protein crystallography is a structural technique that permits the localization of hydrogen atoms, thereby providing important mechanistic details of protein function. We present here the workflow for mounting a protein crystal, neutron diffraction data collection, structure refinement and analysis of the neutron scattering length density maps.
Tags
Vídeos Relacionados
Analyzing Protein Dynamics Using Hydrogen Exchange Mass Spectrometry
Supercritical Nitrogen Processing for the Purification of Reactive Porous Materials
Functionalization of Single-walled Carbon Nanotubes with Thermo-reversible Block Copolymers and Characterization by Small-angle Neutron Scattering
X-ray Powder Diffraction in Conservation Science: Towards Routine Crystal Structure Determination of Corrosion Products on Heritage Art Objects
Highly Stable, Functional Hairy Nanoparticles and Biopolymers from Wood Fibers: Towards Sustainable Nanotechnology
Synthesis of Protein Bioconjugates via Cysteine-maleimide Chemistry
Microfluidic Chips for In Situ Crystal X-ray Diffraction and In Situ Dynamic Light Scattering for Serial Crystallography
Experimental Methods for Efficient Solar Hydrogen Production in Microgravity Environment
Synthesis of Metal Nanoparticles Supported on Carbon Nanotube with Doped Co and N Atoms and its Catalytic Applications in Hydrogen Production
Hydrogen Production and Utilization in a Membrane Reactor
SOBRE A JoVE
Copyright © 2024 MyJoVE Corporation. Todos os direitos reservados