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Preparation of Acute Slices from Dorsal Hippocampus for Whole-Cell Recording and Neuronal Reconstruction in the Dentate Gyrus of Adult Mice

DOI :

10.3791/61980-v

10:45 min

April 3rd, 2021

April 3rd, 2021

6,488 Views

1Institute of Physiology, Christian-Albrechts-University Kiel

We present a protocol to prepare acute-slices from the dorsal-intermediate hippocampus of mice. We compare this transversal preparation with coronal slicing in terms of quality of recordings and preservation of morphological features of recorded neurons.

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Acute Slices

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