Results: Enrichment, Expansion, and Characterization of iNKT Cells
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Conclusion
Transcrição
This protocol enables the purification of INK T-cells, which are very rare, and allows a 30-fold expansion in their number. The purification can be performed in one day and produce a large number of ready-to-use INK T-cells for in vivo and in vitr
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We describe a rapid and robust protocol to enrich invariant natural killer T (iNKT) cells from mouse spleen and expand them in vitro to suitable numbers for in vitro and in vivo studies.