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JoVE Journal

Immunology and Infection

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Purification and Expansion of Mouse Invariant Natural Killer T Cells for in vitro and in vivo Studies

3.8K Views

08:37 min

February 15th, 2021

February 15th, 2021

3,846 Views

0:04

Introduction

0:39

Spleen Processing

1:29

T Cell Enrichment

3:27

iNKT Cell Enrichment

5:21

iNKT Cell Activation and Expansion

6:35

Results: Enrichment, Expansion, and Characterization of iNKT Cells

7:50

Conclusion

Transcript

This protocol enables the purification of INK T-cells, which are very rare, and allows a 30-fold expansion in their number. The purification can be performed in one day and produce a large number of ready-to-use INK T-cells for in vivo and in vitr

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We describe a rapid and robust protocol to enrich invariant natural killer T (iNKT) cells from mouse spleen and expand them in vitro to suitable numbers for in vitro and in vivo studies.

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