apoptosis-induction-detection-primary-culture-sea-cucumber-intestinal

Apoptosis Induction and Detection in a Primary Culture of Sea Cucumber Intestinal Cells

Primary cultured cells are used in a variety of scientific disciplines as exceptionally important tools for the functional evaluation of biological ...

Zhejiang Ocean University

Interleukin-8 (IL-8 or chemokine (C-X-C motif) ligand 8, CXCL8) is a chemokine produced by multiple cell types. It promotes chemotaxis and phagocytosis via interaction with chemokine receptors CXCR1 and CXCR2. Using published data, IL-8 gene (LcIL-8) of the large yellow croaker (Larimichthys crocea) was cloned into the pcDNA3.1 plasmid, and an interleukin-8 receptor (LcCXCR2) was cloned into the pEGFP-N1 plasmid. Secratory expression of LcIL-8 in HEK293T cells was carried out, and product in culture medium was collected for LcCXCR2 stimulation in HEK293 cells. Following receptor internalization observation and intracellular signaling detection, the functional interaction of LcIL-8 and LcCXCR2 was further determined and the ERK phosphorylation signal activation mediated by LcCXCR2 was demonstrated. Quantitative real-time PCR analysis was used to analyze transcription level regulation of LcIL-8 and LcCXCR2 in various tissues of large yellow croaker. Expression of LcIL-8 and LcCXCR2 was elevated in the spleen, head kidney, and liver after Vibrio parahemolyticus challenge. Results illustrated the functional interaction between LcIL-8 and LcCXCR2 in mediating intracellular ERK1/2 phosphorylation signaling and suggested that the LcIL-8 and LcCXCR2 system is part of the immune response induced by V. Parahemolyticus in L. crocea.

Xu Chen

National Engineering Research Center of Marine Facilities Aquaculture,
Marine Science College,
National Engineering Research Center of Marine Facilities Aquaculture, Marine Science College

Zhejiang Ocean University

Apoptosis Induction and Detection in a Primary Culture of Sea Cucumber Intestinal Cells

Xu Chen

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Zhejiang Ocean University

Apoptosis Induction and Detection in a Primary Culture of Sea Cucumber Intestinal Cells

National Engineering Research Center of Marine Facilities Aquaculture,
Marine Science College,
National Engineering Research Center of Marine Facilities Aquaculture, Marine Science College