isolation-mouse-kidney-resident-cd8-t-cells-for-flow-cytometry

Isolation of Mouse Kidney-Resident CD8<sup>+</sup> T cells for Flow Cytometry Analysis

Tissue-resident memory T cell (TRM) is a rapidly expanding field of immunology research. Isolating T cells from various non-lymphoid tissues is one of the ...

University of Texas Health Science Center at San Antonio

The long-term maintenance of memory T cells is essential for successful vaccines. Both the quantity and the quality of the memory T-cell population must be maintained. The signals that control the maintenance of memory T cells remain incompletely identified. Here we used two genetic models to show that continuous transforming growth factor-β signaling to antigen-specific T cells is required for the differentiation and maintenance of memory CD8(+) T cells. In addition, both infection-induced and microbiota-induced inflammation impact the phenotypic and functional identity of memory CD8(+) T cells. 

Transforming growth factor-β signaling is constantly shaping memory T-cell population.

Tissue-resident memory T (TRM) cells have emerged as a major component of T cell biology. Recent investigations have greatly advanced our understanding of TRMs. Common features have been discovered to distinguish memory T cells residing in various mucosal and non-mucosal tissues from their circulating counterparts. Given that most organs and tissues contain a unique microenvironment, local signal-induced tissue-specific features are tightly associated with the differentiation, homeostasis, and protective functions of TRMs. Here, we discuss recent advances in the TRM field with a special emphasis on the interaction between local signals and TRMs in the context of individual tissue environment.

Chaoyu Ma

Department of Microbiology,
Immunology and Molecular Genetics,
School of Medicine,
Department of Microbiology, Immunology and Molecular Genetics, School of Medicine

University of Texas Health Science Center at San Antonio

Isolation of Mouse Kidney-Resident CD8⁺ T cells for Flow Cytometry Analysis

Chaoyu Ma

.css-o250i3{font-size:18px;font-family:Open Sans,sans-serif;line-height:21.6px;}Department of Microbiology,Immunology and Molecular Genetics,School of Medicine,Department of Microbiology, Immunology and Molecular Genetics, School of Medicine

University of Texas Health Science Center at San Antonio

Isolation of Mouse Kidney-Resident CD8+ T cells for Flow Cytometry Analysis

Department of Microbiology,
Immunology and Molecular Genetics,
School of Medicine,
Department of Microbiology, Immunology and Molecular Genetics, School of Medicine

Isolation of Mouse Kidney-Resident CD8⁺ T cells for Flow Cytometry Analysis