automated-dissection-protocol-for-tumor-enrichment-low-tumor-content

Automated Dissection Protocol for Tumor Enrichment in Low Tumor Content Tissues

Tumor enrichment in low tumor content tissues, those below 20% tumor content depending on the method, is required to generate quality data reproducibly ...

synthetic-antigen-controls-for-immunohistochemistry

Synthetic Antigen Controls for Immunohistochemistry

Immunohistochemistry (IHC) assays provide valuable insights into protein expression patterns, the reliable interpretation of which requires ...

Genentech

Genentech, Inc.

Optimization and standardization of immunohistochemistry (IHC) protocols within and between laboratories requires reproducible positive and negative control samples. In many situations, suitable tissue or cell line controls are not available. We demonstrate here a method to incorporate target antigens into synthetic protein gels that can serve as IHC controls. The method can use peptides, protein domains, or whole proteins as antigens, and is compatible with a variety of fixation protocols. The resulting gels can be used to create tissue microarrays (TMAs) with a range of antigen concentrations that can be used to objectively quantify and calibrate chromogenic, fluorescent, or mass spectrometry-based IHC protocols. The method offers an opportunity to objectively quantify IHC staining results, and to optimize and standardize IHC protocols within and between laboratories. (J Histochem Cytochem 58:XXX-XXX, 2019).

Charles A. Havnar

Departments of Research Pathology

Genentech

Automated Dissection Protocol for Tumor Enrichment in Low Tumor Content Tissues

Synthetic Antigen Controls for Immunohistochemistry

Charles A. Havnar

.css-o250i3{font-size:18px;font-family:Open Sans,sans-serif;line-height:21.6px;}Departments of Research Pathology

Genentech

Automated Dissection Protocol for Tumor Enrichment in Low Tumor Content Tissues

Synthetic Antigen Controls for Immunohistochemistry

Departments of Research Pathology