Epithelial Effects of Intestinal Inflammation on Established Murine Colonoids

June 2nd 2023


* Эти авторы внесли равный вклад


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We describe a protocol detailing the isolation of murine colonic crypts for the development of 3-dimensional colonoids. The established colonoids can then be terminally differentiated to reflect the cellular composition of the host epithelium prior to receiving an inflammatory challenge or being directed to establish an epithelial monolayer.

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