Yokohama City University Graduate School of Medical Life Science

1 ARTICLES PUBLISHED IN JoVE

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Neuroscience

Presynapse Formation Assay Using Presynapse Organizer Beads and “Neuron Ball” Culture
Shumaia Parvin 1, Renoma Takeda 1, Yukio Sasaki 1
1Functional Structure Biology Laboratory, Department of Medical Life Science, Yokohama City University Graduate School of Medical Life Science

Presynapse formation is a dynamic process including accumulation of synaptic proteins in proper order. In this method, presynapse formation is triggered by beads conjugated with a presynapse organizer protein on axonal sheets of “neuron ball” culture, so that accumulation of synaptic proteins is easy to be analyzed during presynapse formation.

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