Brigham and Women's Hospital-Harvard Medical School

1 ARTICLES PUBLISHED IN JoVE

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Genetics

Characterization of Functionally Associated miRNAs in Glioblastoma and their Engineering into Artificial Clusters for Gene Therapy
Vivek Bhaskaran 1, Pierpaolo Peruzzi 1
1Department of Neurosurgery, Brigham and Women's Hospital-Harvard Medical School

Described here is a protocol for characterizing modules of biologically synergistic miRNAs and their assembly into short transgenes, which allows simultaneous overexpression for gene therapy applications.

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