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Chapter 12

Introduction to Molecular Spectroscopy

Dual Nature of Electromagnetic (EM) Radiation
Dual Nature of Electromagnetic (EM) Radiation
Electromagnetic (EM) radiation consists of electric and magnetic field components oscillating in planes perpendicular to each other and mutually ...
Interaction of EM Radiation with Matter: Spectroscopy
Interaction of EM Radiation with Matter: Spectroscopy
Electromagnetic (EM) radiation can be considered an oscillating electric and magnetic field propagating through a medium that can interact with matter in ...
Molecular Spectroscopy: Absorption and Emission
Molecular Spectroscopy: Absorption and Emission
Molecules possess discrete energy levels called quantum states. Unlike atoms, which have simpler energy levels, molecules possess additional rotational ...
Spectrophotometry: Introduction
Spectrophotometry: Introduction
Spectrophotometry is the quantitative measurement of the absorption, reflection, diffraction, or transmission of electromagnetic radiation through a ...
Ultraviolet and Visible (UV–Vis) Spectroscopy: Overview
Ultraviolet and Visible (UV–Vis) Spectroscopy: Overview
Ultraviolet–visible (UV–visible or UV–Vis) spectroscopy is an analytical technique that investigates the interaction between matter and ...
UV–Vis Spectroscopy: Molecular Electronic Transitions
UV–Vis Spectroscopy: Molecular Electronic Transitions
In Ultraviolet–Visible (UV–Vis) spectroscopy, the absorption of electromagnetic radiation is used to probe the electronic structure of ...
UV–Vis Spectrometers
UV–Vis Spectrometers
The absorbance of UV and visible (UV–visible) radiations is measured using a UV–visible spectrophotometer. Deuterium lamps, which emit UV ...
UV–Vis Spectrum
UV–Vis Spectrum
When light passes through a substance, a portion of the light is absorbed while the remaining light is reflected or transmitted. If the molecule absorbs ...
UV–Vis Spectroscopy: Beer–Lambert Law
UV–Vis Spectroscopy: Beer–Lambert Law
The Beer-Lambert law describes the relationship between absorbance and concentration, which combines the principles established by scientists Johann ...
Photoluminescence: Fluorescence and Phosphorescence
Photoluminescence: Fluorescence and Phosphorescence
Photoluminescence is a process where a molecule absorbs light energy and re-emits it in the form of light. This phenomenon occurs when a substance absorbs ...
Variables Affecting Phosphorescence and Fluorescence
Variables Affecting Phosphorescence and Fluorescence
Fluorescence and phosphorescence are essential phenomena in fields like analytical chemistry, biological imaging, and materials science, where they detect ...
Deactivation Processes: Jablonski Diagram
Deactivation Processes: Jablonski Diagram
Luminescence, the emission of light by a substance that has absorbed energy, is a process that involves the interaction of molecules with light. The ...
Photoluminescence: Applications
Photoluminescence: Applications
Photoluminescence offers a wide range of applications due to its inherent sensitivity and selectivity. This technique allows for both direct and indirect ...
Fluorescence and Phosphorescence: Instrumentation
Fluorescence and Phosphorescence: Instrumentation
Fluorometers and spectrofluorometers are two types of instruments used for measuring molecular fluorescence. These instruments differ in how they select ...
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