The nervous system consists of complex motor neuron circuits, including upper motor neurons originating from the cerebral cortex and lower motor neurons starting in the spinal cord, coordinating both voluntary and involuntary movements. Among these, somatic motor neurons activate skeletal muscles and are classified into alpha, beta, and gamma types. Alpha neurons are vital for voluntary movement coordination, while gamma neurons adjust muscle spindle sensitivity, and the function of beta neurons is less understood. These neurons form a critical two-neuron circuit for body movement control.

At the heart of this circuit lies the neuromuscular junction (NMJ), a specialized synapse where motor neurons communicate with skeletal muscle fibers, enabling movement and muscular contraction. This junction is not merely a physical link but a dynamic, biochemical interface ensuring precise control over muscle function. The presynapse of the NMJ refers to the end of the somatic motor neuron that connects to a muscle fiber. It includes the axon terminal and synaptic end bulbs filled with synaptic vesicles. These vesicles contain acetylcholine, a neurotransmitter that is crucial for muscle contraction.

The synaptic cleft is a tiny gap that separates the neuron from the muscle fiber. This space is filled with a gel-like extracellular substance rich in glycoproteins and collagen fibers, facilitating neurotransmitter diffusion to the post-synapse. It also contains the enzyme acetylcholinesterase that can hydrolyze acetylcholine and, as a result, regulates the amount of neurotransmitter that reaches the postsynaptic receptors.

Lastly, the post-synapses of a neuromuscular junction refer to the part of the muscle fiber called the motor end plate that receives the signal from the neuron. The motor end plate has modified sarcolemma with junctional folds that increase the surface area for the placement of acetylcholine receptors.