JoVE Encyclopedia of Experiments
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Overview
Encyclopedia of Experiments
CRC Organoid Culture: A Method to Obtain 3D Organoids from Colorectal Cancer Cells
Overview
This video describes the detailed protocol for generating 3D organoids from murine-model derived colorectal cancer cells by propagating them on artificial extracellular matrix coated plates. These CRC organoids can be used as a model to study the primary growth of human tumor cells and metastasis.
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Source: Okazawa, Y. et al. High-sensitivity Detection of Micrometastases Generated by GFP Lentivirus-transduced Organoids Cultured from a Patient-derived Colon Tumor. J. Vis. Exp., (2018).
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