eoe sub articles

EoE Sub Articles

All procedures involving animal models have been reviewed by the local institutional animal care committee and the JoVE veterinary review board.
1. Harvest and Preparation of DRGs (estimated timing: 2 h, day 8)
NOTE: Experiments with mice and rats require approval from the IACUC. In some countries, the use of chicken eggs also requires approval.
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	<li>Get six to seven week-old Sprague Dawley rats (~200 g in weight) to extract ...

harvesting drg from rat spine: a procedure to extract dorsal root ganglion from the spine of a rat model

Source: Schmidt, L. B. et al. <a href="https://www.jove.com/video/59296" target="_blank">The Chick Chorioallantoic Membrane In Vivo Model to Assess Perineural Invasion in Head and Neck Cancer.</a> J. Vis. Exp. (2019)
In this video, we demonstrate the extraction of dorsal root ganglion or DRG from a harvested rat spine for downstream applications.

<img alt="Figure 1" class="xfigimg" src="/files/ftp_upload/20476/20476fig1.jpg" /> 
Figure 1: DRG extraction on day 8. A. Rat schematic illustrating the anatomical location of the spine. B. Diagram of the rat vertebrae configuration showing different body regions; green for cervical, dark blue for thoracic, orange for lumbar and light blue for sacral vertebrae. C-D. Ventral aspect of the rat spine after surgical exci...

Harvesting DRG from Rat Spine: A Procedure to Extract Dorsal Root Ganglion from the Spine of a Rat Model

Source: Schmidt, L. B. et al. The Chick Chorioallantoic Membrane In Vivo Model to Assess Perineural Invasion in Head and Neck Cancer. J. Vis. Exp. (2019)
...

The dorsal root ganglia or DRG are clusters of cell bodies of sensory neurons lodged within the vertebrae on either side of the spinal cord. Their primary function is to transmit sensory neuronal signals from the peripheral nervous system to the central nervous system.
To isolate the dorsal root ganglia, begin by taking a freshly harvested rat spine. Dissect the spine to separate the cervical, thoracic, and lumbar regions. Keep the spine sections in a&#160; suitable buffer to ensure tissue hydration.
Now, position the thoracic section with its dorsal side facing up. Divide the section into two lateral halves. Next, secure the dissected half of the spine segment, medial side up. Gently detach the bisected spinal cord - a long, tubular structure - from the segmented vertebral bones of the section to reveal the underlying DRG.
The DRG appear as distinct white masses with dorsal and ventral roots embedded within the spinal canal. With fine forceps, carefully pull out the DRG to dislodge it from the spinal canal. Finally, transfer the harvested DRG into a suitable media for further downstream applications.

Harvesting DRG from Rat Spine: A Procedure to Extract Dorsal Root Ganglion from the Spine of a Rat Model

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