Structure-function Studies in Mouse Embryonic Stem Cells Using Recombinase-mediated Cassette Exchange

Summary

Proteins often contain multiple domains that can exert different cellular functions. Gene knock-outs (KO) do not consider this functional diversity. Here, we report a recombination-mediated cassette exchange (RMCE)-based structure-function approach in KO embryonic stem cells that allows for the molecular dissection of various functional domains or variants of a protein.

Abstract

Gene engineering in mouse embryos or embryonic stem cells (mESCs) allows for the study of the function of a given protein. Proteins are the workhorses of the cell and often consist of multiple functional domains, which can be influenced by posttranslational modifications. The depletion of the entire protein in conditional or constitutive knock-out (KO) mice does not take into account this functional diversity and regulation. An mESC line and a derived mouse model, in which a docking site for FLPe recombination-mediated cassette exchange (RMCE) was inserted within the ROSA26 (R26) locus, was previously reported. Here, we report on a structure-function approach that allows for molecular dissection of the different functionalities of a multidomain protein. To this end, RMCE-compatible mice must be crossed with KO mice and then RMCE-compatible KO mESCs must be isolated. Next, a panel of putative rescue constructs can be introduced into the R26 locus via RMCE targeting. The candidate rescue cDNAs can be easily inserted between RMCE sites of the targeting vector using recombination cloning. Next, KO mESCs are transfected with the targeting vector in combination with an FLPe recombinase expression plasmid. RMCE reactivates the promoter-less neomycin-resistance gene in the ROSA26 docking sites and allows for the selection of the correct targeting event. In this way, high targeting efficiencies close to 100% are obtained, allowing for insertion of multiple putative rescue constructs in a semi-high throughput manner. Finally, a multitude of R26-driven rescue constructs can be tested for their ability to rescue the phenotype that was observed in parental KO mESCs. We present a proof-of-principle structure-function study in p120 catenin (p120ctn) KO mESCs using endoderm differentiation in embryoid bodies (EBs) as the phenotypic readout. This approach enables the identification of important domains, putative downstream pathways, and disease-relevant point mutations that underlie KO phenotypes for a given protein.

Introduction

It is estimated that mammalian genomes contain about 20,000 protein-coding genes. Alternative splicing and posttranslational modifications further increase the protein repertoire. Proteins have a modular structure¹ and often contain multiple interaction domains, which allow their recruitment into different protein complexes and their participation in multiple cellular processes². One example is the multi-functional protein called p120ctn. p120ctn is encoded by the Ctnnd1 gene and consists of a large central armadillo repeat domain flanked by an N-terminal and a C-terminal region. The armadillo domain of p120ctn binds to a highly conserved juxtamembrane domain of classical cadherins, which are involved in cell-cell adhesion, but it also binds to the transcriptional repressor Kaiso. The N-terminal domain of p120ctn interacts with different kinases, phosphatases, small RhoGTPases, and microtubule-associated proteins³. Interestingly, as a result of alternative splicing, p120ctn isoforms can be generated from four alternative start codons⁴. p120ctn isoform 1A is the longest, as it is translated from the most-5' start codon and contains the full-length N-terminal segment. In p120ctn isoforms 3 and 4, this N-terminal segment is deleted partially and completely, respectively. Understanding the precise role of proteins (or protein isoforms) and their domains in different cellular functions remains a challenge.

Gene targeting in mESCs enables the study of the function of a protein through the genetic deletion of the corresponding gene and has widely contributed to the identification of developmentally important and disease-relevant genes and pathways. This breakthrough in reverse genetics was the result of advances in the fields of mESC isolation and gene targeting due to homologous recombination⁵. Homologous recombination is a process in which DNA fragments are exchanged between two similar or identical nucleic moieties after double-stranded (ds) DNA breaks. Normally, HR is inefficient because dsDNA breaks are infrequent. Recently, the efficiency of homology-directed gene targeting could be increased using site-specific nucleases^6,7, but unfortunately, these are prone to off-target effects⁸. A more reliable technique to enable gene targeting is RMCE, which is based on site-specific recombination systems such as Cre/loxP or FLPe/Frt. LoxP and Frt sequence are found in bacteriophage P1 and Saccharomyces cerevisiae, respectively, and consist of 34 bp, including an asymmetric 8 bp sequence that determines the orientation of the site. On the other hand, the orientation of, for instance, two loxP sites within a DNA stretch will determine whether the floxed DNA becomes excised or inversed upon Cre-mediated recombination⁹. Moreover, Cre can also induce a translocation if two sites are located on different chromosomes. RMCE takes advantage of heterospecific recombination sites that do not cross-react and that are embedded in a genomic locus. In the presence of a donor plasmid that contains a DNA fragment flanked by the same heterospecific sites, the recombinase will insert this DNA fragment into the RMCE-compatible genomic locus because of double-simultaneous translocation (Figure 1). Here, only correctly RMCE-targeted clones can render drug resistance thanks to a promoter on the incoming vector that restores a "trapped," promoter-less Neomycin resistance gene (Neo^R) present in the R26 genome of the docking cells (Figure 1)^10,11. This results in a very high targeting efficiency, often close to 100%^11,12. In conclusion, RMCE-based targeting is highly efficient and can be used for structure-functions studies; however, it requires a pre-engineered genomic locus.

Figure 1. Schematic Representation of RMCE-mediated Targeting. RMCE allows for the exchange of DNA segments from an incoming targeting vector to a defined genomic locus if both harbor two heterospecific Frt sites (depicted by white and red triangles). In addition, the engineered genomic locus contains a promoterless and truncated neomycin-resistance (Neo^R) gene. By providing a promoter and start codon in the incoming DNA fragment, only correct recombination events restore neomycin resistance, resulting in high targeting efficiencies. Please click here to view a larger version of this figure.

Genome engineering in mESCs allows for the generation of RMCE-compatible mice. In 1981, two groups succeeded in capturing pluripotent cells from the inner cell mass (ICM) of blastocysts and in maintaining them in culture^13,14. mESCs are capable of self-renewal and differentiation into all types of embryonic and adult cells, including the germ-cell lineage. Therefore, gene targeting in mESCs enables reverse-genetic studies through the development of constitutive or conditional (using the Cre/LoxP system) KO mice. However, the classical way to isolate mouse ES cells is very inefficient. Several major improvements have greatly increased the success rate for deriving mESC lines, including the use of a defined serum-replacement (SR) medium¹⁵, alternating between mESC medium containing SR and fetal bovine serum (FBS)¹⁶, and the use of pharmacological compounds such as pluripotin or 2i¹⁷. Pluripotin, a small synthetic molecule, allows for the propagation of mESCs in an undifferentiated state in the absence of leukemia inhibitory factor (LIF) and mouse embryonic fibroblasts (MEFs)¹⁸. Finally, it has been shown that mESCs can be isolated with a very high efficiency (close to 100%) when an SR/FBS medium alternation protocol is combined with LIF and pluripotin^19,20. These protocols enable the efficient isolation of RMCE-compatible KO mESCs that can subsequently be used for structure-function studies.

This paper describes a method that enables one to identify the key domains or residues within a protein that are responsible for specific cellular processes. To this end, a pipeline of advanced technologies that enable efficient mESC isolation, targeting vector assembly, and mESC targeting was created. As such, large panels with protein isoforms, domain mutants, and downstream effectors can be introduced in KO mESCs and can be evaluated for their ability to rescue the in vitro KO phenotype.

Protocol

All experiments on mice were conducted according to institutional, national, and European animal regulations.

1. Isolation of RMCE-compatible KO mESCs

1. Breed heterozygous KO mice with RMCE-compatible mice, such as ROSALUC mice¹⁰ or ROSA26-iPSC mice²¹. Both RMCE-compatible mice were maintained on a mixed 129/C57BL6/Swiss background.
   NOTE: Crossing with heterozygous KO mice is advised to overcome embryonic lethality in homozygous KO mice.
2. Use PCR to select heterozygous KO mice containing an RMCE cassette in the R26 locus¹².
3. Breed RMCE-compatible, heterozygous KO mice with heterozygous KO mice and isolate RMCE-compatible, homozygous KO blastocysts.
   1. Set up time matings in the evening and check for copulation plugs the next morning.
      NOTE: Plugs are made of coagulated secretions from the coagulating and vesicular glands of the male. These plugs fill the vagina of the female and persist for 8 - 24 h after breeding. Plugged females are considered to be carrying 0.5 dpc (days post coitum) embryos.
      1. To check for plugs, lift the female by the base of her tail and by examine her vaginal opening for a whitish mass. Spread the lips of the vulva slightly with an angled probe when the plug is difficult to see. Separate plugged females from their male.
   2. Collect blastocysts at 3.5 dpc.
      1. Euthanize pregnant females by the approved method (e.g., cervical dislocation). Make a midventral incision and dissect the uterus and oviduct (still attached to each other) using fine scissor and forceps.
      2. Bend a 26-gauge needle into a 45° angle. Attach a 1-mL syringe filled with M2 medium to this bent needle and use it to flush the blastocysts from the uterus into the lid of a 10-cm dish.
         1. Insert the needle into the end of the uterus that is closest to the oviduct. Hold the needle in place with fine forceps while pushing the plunger; swelling of the uterus indicates a successful flushing.
      3. Use a mouth pipette (with a diameter of 100 - 200 µm) to collect all embryos and wash them twice in a drop of fresh M2 medium. Immediately after washing them, transfer the blastocysts to the culture plates (see below).
         NOTE: The dissection and handling of blastocysts of should be done in laminar air flow.
4. Isolate RMCE-compatible KO mESCs
   1. Prepare a 12-well plate with mitomycin-C-treated DR4 MEFs (see the Table of Materials) one day before the blastocyst isolation.
      NOTE: These MEFs were isolated from Tg(DR4)1Jae/J mice that contain four drug-selectable genes and confer resistance to neomycin, puromycin, hygromycin, and 6-thioguanine²².
      1. Coat all culture plates with 0.1% gelatin. Add 0.1% gelatin to the culture plates, incubate for 5 min at 37 °C in 5% CO₂, and aspirate the gelatin solution. Seed one quarter of a vial of P2 MEFs in a 12-well plate and grow them in 2 mL of MEF medium (see Table 1, the Table of Materials) to a confluent monolayer¹⁹.
      2. Inactivate them with mitomycin-C (10 µg/mL) for 3 h and wash them twice with phosphate-buffered saline (PBS)¹⁹.
   2. Using a mouth pipette, plate the blastocysts onto gelatinized 12-well plates (1 well/embryo), with the mitomycin-C-treated MEFs in SR-ES cell medium (2 mL/well) supplemented with either 2 µM pluripotin or with 2i (1 µM Erk inhibitor PD0325901 and 3 µM Gsk3 inhibitor CHIR99021). Incubate at 37 °C in 5% CO₂.
   3. Refresh the SR-ES cell medium (supplemented with pluripotin or 2i) every 2 - 3 days.
   4. Examine each blastocyst under a stereomicroscope at 4.0X magnification and check for hatching and attachment to the MEF layer.
      NOTE: When blastocysts hatch, they lose the zona pellucida that encapsulates them. Wells with unattached blastocysts need to be refreshed using the mouth pipetting.
   5. Pick individual ICM outgrowths (using a stereomicroscope) after 10 - 12 days of culture using a P10 pipette with disposable tips. Transfer the outgrowth in approximately 10 µL of medium to a V-shaped, 96-well plate containing 30 µL/well of PBS (at room temperature).
   6. Add 50 µL of 0.25% trypsin to each well using a multichannel pipette and incubate for 3 min at 37 °C in 5% CO₂.
   7. Add 100 µL of FBS-containing mESC medium; dissociate the ICM outgrowths into single cells by pipetting 10-15 times; and transfer the dissociated cells to mitomycin-C-treated, 96-well MEF plates that were prepared one day before the ICM colonies are picked.
   8. From this step onwards, omit pluripotin or 2i from the mESC medium. On the next day, change the medium from FBS- to SR-containing mESC medium (100 µL/well).
   9. Expand the established mESC lines from 96- to 24-well format¹⁹.
      1. Wash the cells with 200 µL of PBS, add 50 µL of trypsin, and incubate for 5 min at 37 °C in 5% CO. Add 100 µL of FBS-based mESC medium; dissociate by pipetting 10 - 15 times using a multichannel pipette; and transfer the dissociated cells to mitomycin-C-treated, 24-well MEF plates.
      2. Change to SR-based medium on the next day. Expand the mESCs in a similar fashion from 24- to 6-well format. Make 3 - 4 freezings from a confluent 6-well plate¹⁹.
   10. Identify RMCE-compatible, homozygous KO mESCs using PCR primers for the R26 locus²³ and KO allele of choice (in this case, p120ctn; PCRs for p120ctn null and floxed alleles were described before¹²).

Table 1. Culture Media. All media were stored at 4 °C and warmed to 37 °C 30 min before use.

2. Generation of an RMCE-compatible Targeting Vector Using Recombination Cloning

1. Clone the rescue constructs into recombination-compatible vectors using restriction enzyme (RE)-based or PCR-based²⁴ cloning techniques. Make sure that the cDNAs contain a stop codon.
   1. Design AttB-tagged primers²⁴. Ensure that the forward primer contains the following elements: a GGGG stretch, an AttB1 site, a linker, a Kozak sequence, and about 25 nucleotides of rescue cDNA (starting with its ATG). Make sure that the reverse primer has a similar composition: a GGGG stretch, an AttB2 site, a linker, and about 25 nucleotides of rescue cDNA (reverse complement).
   2. Amplify the rescue cDNA via PCR to obtain AttB-flanked cDNA.
   3. Perform a 10-µL BP reaction with 100 ng of AttB-flanked cDNA and 150 ng of recombination-compatible donor vector, which contains a kanamycin-resistance gene.
   4. Transform 5 µL of the BP mixtures in heat-shock-competent MC1061 Escherichia coli (E. coli) bacteria (similar to those described in step 2.3).
   5. Identify colonies containing the correct rescue cDNA-containing vectors (similar to those described in step 2.4)
2. Perform a 10-µL LR reaction using 100 ng of rescue cDNA-containing vector; 150 ng of Cre-excised pRMCE-DV1 vector¹¹ (LMBP 8195); and 2 µL of recombinase mix, which contains a phage-encoded integrase and excisionase and a bacterial integration host factor. Incubate for 2 h at 25 °C.
   NOTE: An LR reaction is a recombination reaction in which an entry clone containing attL sites and a destination vector containing attR sites are recombined by the LR clonase enzyme mix. This results in an expression clone containing attB sites flanking the gene of interest.
3. Transform 5 µL of the LR mixtures in heat-shock-competent MC1061 E. coli bacteria.
   1. Add 5 µL of LR mixtures to a ribbed, skirted, 2-mL screw-cap tube with 40 µL of heat-shock-competent E. coli bacteria and incubate for 20 min on ice. Incubate for 5 min at 37 °C.
   2. Add 1 mL of Luria broth (LB) medium and incubate for 1 h at 37 °C. Plate 50 µL on ampicillin (Amp; 100 µg/mL)-containing agar plates and grow overnight at 37 °C.
4. Identify the colonies with the correct targeting vector.
   1. Pick 5 colonies randomly using a p200 tip. Transfer the tip to a glass test tube containing 2 - 5 mL of LB medium and grow overnight at 37 °C.
   2. Extract the plasmid DNA from the bacterial cultures using commercially available kits.
   3. Validate them using RE digests and sequencing. Cut 0.5 - 2 µg of plasmid using 0.2 µL of RE (20 U/µL) and 1 µL of the corresponding 10x buffer in a 10-µL reaction. Incubate for at least 1 h at 37 °C and separate on a 1% agarose gel. Select for colonies with the predicted pattern of DNA fragments.
      1. Analyze the confirmed vectors (50 ng/µL) with Tlox F (ATC ATG TCT GGA TCC CCA TC) and IRES R (GGG GCG GAA TTC GAT ATC AAG) primers (5 pmol/µL) using Sanger sequencing.

3. RMCE-mediated mESC Targeting of Rescue Constructs to the R26 Locus

1. Start a culture of RMCE-compatible KO mESCs and passage them at least twice on MEFs in FBS-based mESC medium. Split the mESCs on a gelatinized 6-well plate.
2. On the next day, refresh the cells, at about 50% confluency, with 1.5 mL of FBS-based mESC medium and transfect the mESCs with a Cre-excised pRMCE-DV1 targeting vector containing rescue cDNA.
   1. Make a DNA mix. Add 1 µg of targeting vector and 1 µg of FLPe-expression plasmid²⁵ to 250 µL of pure DMEM medium.
   2. Make a lipofection mix. Add 7 µL of lipofection-based transfection reagent (e.g., Lipofectamine 2000) to 250 µL of pure DMEM medium and incubate for 5 min at room temperature (RT).
      NOTE: Similar RMCE targeting efficiencies were obtained using other lipofection-based reagents (e.g., Lipofectamine LTX and Effectene).
   3. Mix the DNA mix with the lipofection mix and incubate for 20 min at RT. Pipette the transfection mixture onto the refreshed mESCs. Swirl gently and leave overnight.
3. One day after transfection, split all mESCs from the 6-well plate to a 10-cm culture dish with a confluent layer of DR4 MEFs and 10 mL of FBS-based mESC medium.
4. Two days after transfection, select mESC clones with the correct FLPe-mediated cassette exchange by adding 0.2 mg/mL G418 (100x) to the medium.
   NOTE: Make a kill curve for each batch of G418 to identify the lowest concentration of G418 that kills all mESCs.
5. Refresh the mESCs daily with G418-containing mESC medium. Colonies should appear after 7 - 10 days, so pick and expand these as per step 1.4.
6. Confirm the correct clones via PCR¹¹.

4. Differentiation of mESCs in Embryoid Bodies (EBs)

1. Start a culture of KO mESCs with R26-driven rescue constructs and passage them at least twice on MEFs in FBS-based mESC medium. Passage the mESCs once on gelatinized 6-well plates to get rid of the MEFs.
2. Wash with PBS and trypsinize the nearly-confluent mESC cultures. Plate dissociated mESCs in different dilutions (1/20 and 1/40) onto non-adherent, bacterial-grade petri-dishes in differentiation medium.
3. Allow EBs to form in these dishes for 30 days. Refresh the medium every 2 - 3 days using the following procedure: transfer the EB suspension to a 50-mL tube, let the EBs settle by gravity, remove the supernatant, add fresh medium, and transfer the EB suspension back to a bacterial-grade dish.
4. Analyze the targeted mESCs and EBs by immunofluorescence and transmission electron microscopy using protocols that were described previously¹².

Results

The procedure to isolate RMCE-compatible KO mESC lines is depicted in Figure 2. Two consecutive breedings are required to obtain RMCE-compatible KO blastocysts. First, heterozygous KO mice are crossed with RMCE-compatible mice to obtain RMCE-compatible, heterozygous KO mice. These mice are then used for timed matings with other heterozygous KO mice to obtain 3.5-dpc, RMCE-compatible, homozygous KO blastocysts. The chance of obtaining such an embryo is one in eight, as pre...

Discussion

Our mESC isolation method is user-friendly and does not require advanced skills or equipment, such as microsurgery of blastocysts. Thus, this technology is accessible to a large proportion of the scientific community. Anyone with basic cell culture experience can propagate ICM outgrowths and establish mESCs lines. However, the flushing and handling of blastocysts requires some practice. A mouth pipette is used to transfer blastocysts and consists of a micropipette, a micropipette holder, tubing, and an aspirator mouthpie...

Materials

Name	Company	Catalog Number	Comments
ROSALUC Mice	made in house		frozen sperm available upon request
R26-iPSC mice	made in house		frozen sperm available upon request
Vessel probe	Fine Science Tools	10160-13	to check for copulation plugs
M2 medium	Sigma-Aldrich	M7167	make aliquots and store at -20 °C
Fine forceps (Dumont #5 Standard tip Student forceps)	Fine Science Tools	11251-10	spray with 70% EtOH before use (do not autoclave)
23 G needles	Fine-ject	8697
1-mL syringes	Soft-ject	6680
60-mm bacterial grade plates (for flushing)	Gosselin	BB60-01
Mouth pipette	made in house		see discussion
Mouse embryonic fibroblasts (MEFs, TgN (DR4)1 Jae strain)	ATTC	SCRC-1045
TgN (DR4)1 Jae mice	The Jackson Laboratory	3208
Mitomycin C	Sigma-Aldrich	M0503
Phosphate buffered saline (PBS) without calcium or magnesium	Gibco	14190-094
Tg(DR4)1Jae/J mice	JAX	3208	mice that contain four drug-selectable genes and DR4 MEFS confers resistance to neomycin, puromycin, hygromycin and 6-thioguanine
0.1% Gelatin	Sigma-Aldrich	G1393	Dissolve 0.5 g in 500 mL distilled water, autoclave and store at 4 °C.
Trypsin (0.25%)	Gibco	25200-056
2 μM pluripotin	Cayman Chemical	10009557
pRMCE-DV1	BCCM/LMBP collection	LMBP 08870	public available from the BCCM/LMBP collection (http://bccm.belspo.be)
cre-excised pRMCE-DV1	BCCM/LMBP collection	LMBP 08195	public available from the BCCM/LMBP collection (http://bccm.belspo.be)
pCAG-FlpE-IRES-Puro-pA	Addgene	20733
heat-shock competent DH5α bacteria	made in house
Gateway pDONR221 vector	Thermo Fisher	12536-017	contains kanamycin-resistance gene
BP clonase II mix	Thermo Fisher	11789-020
LR clonase II mix	Thermo Fisher	11791-020
Luria Broth (LB)
Ampicillin
Applied Biosystems 3730XL DNA Analyzer	Thermo Fisher	3730XL
G418	Thermo Fisher	11811-023
Lipofectamine 2000 transfection reagent	Thermo Fisher	11668027
Lipofectamine LTX transfection reagent	Thermo Fisher	15338100
Effectene transfection reagent	Qiagen	301425
GATEWAY pENTR 1A vector	Thermo Fisher	A10462	recombination-compatible vector
mouse monoclonal anti-p120ctn antibody	BD Transduction Laboratories	610134
mouse monoclonal anti-Ecadherin antibody	BD Transduction Laboratories	610181
General equipment
Sterile dissection tools			fine scissors and forceps for dissecting the uterus
Sterile pipettes: 5 mL, 10 mL and 25 mL
15-mL and 50-mL conical centrifuge tubes
96-well culture plates V-shaped bottom and flat bottom)
Culture dishes: 24 wells, 12 wells and 6 wells
Multichannel pipettes (to pipette 30, 50, 100 and 200 μL)
Sterile multichannel reservoirs
Access to a laminar air flow
Access to an incubator at 37 °C with 5% CO2
Access to an inverted microscope
Access to a bench-top centrifuge
Access to a stereo microscope with transmitted-light
Culture media
MEF Medium			stored at 4 °C; warm 30 min at 37 °C before use
Dulbecco’s modified Eagle’s medium (DMEM)	Gibco	41965-062
10% fetal bovine serum (FBS)	Sigma-Aldrich	F-7524
L-glutamine (2 mM)	Gibco	25030-024
Sodium pyruvate  (0.4 mM)	Gibco	11360-039
penicillin (100 U/mL)	Gibco	15140-122
streptomycin (100 µg/mL)	Gibco	15140-122
SR-based mESC medium			stored at 4 °C; warm 30 min at 37 °C before use
DMEM/F12	Gibco	31330-038	mixed in a 1:1 ratio
15% knock-out serum replacement (SR)	Gibco	10828–028
L-glutamine (2 mM)	Gibco	25030-024
0.1 mM non-essential amino acids	Gibco	11140-050
penicillin (100 U/mL)	Gibco	15140-122
streptomycin (100 µg/mL)	Gibco	15140-122
β-mercaptoethanol (0.1 mM)	Sigma-Aldrich	M 3148
2,000 U/mL recombinant mouse LIF	(IRC/VIB Protein Service facility)
FBS-based mESC medium (similar to SR-based mESC medium)			stored at 4°C; warm 30 min at 37°C before use
Knockout DMEM	Gibco	10829-018
15% FBS	Hyclone	SH30070.03E
Differention Medium			stored at 4 °C; warm 30 min at 37 °C before use
Iscove's Modified Dulbecco's Medium (IMDM)	Gibco	21980-032
15% FBS	Hyclone	SH30070.03E
5% CD Hybridoma Medium(1x) liquid	Gibco	11279-023
2 mM L-glutamine	Gibco	25030-024
0.4 mM 1-thioglycerol	Sigma-Aldrich	M-6145
50 μg/mL ascorbic acid	Sigma-Aldrich	A-4544
penicillin (100 U/mL)	Gibco	15140-122
streptomycin (100 µg/mL)	Gibco	15140-122
2i
1 μM Erk inhibitor PD0325901	Axon Medchem	Axon 1408
3 μM Gsk3 inhibitor CHIR99021	Axon Medchem	Axon 1386