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Abstract

Medicine

An Enzyme-free Method for Isolation and Expansion of Human Adipose-derived Mesenchymal Stem Cells

Published: December 16th, 2019

DOI:

10.3791/59419

1Department of Medicine, Division of Hematology/Oncology, New Jersey Medical School, Rutgers Biomedical and Health Sciences, 2Rutgers School of Graduate Studies at New Jersey Medical School, Rutgers Biomedical and Health Sciences, 3Department of Surgery, Division of Plastic Surgery, New Jersey Medical School, Rutgers Biomedical and Health Sciences

Abstract

Mesenchymal stem cells (MSCs) are a population of multipotent cells that can be isolated from various adult and fetal tissues, including adipose tissue. As a clinically relevant cell type, optimal methods are needed to isolate and expand these cells in vitro. Most methods to isolate adipose-derived MSCs (ADSCs) rely on harsh enzymes, such as collagenase, to digest the adipose tissue. However, while effective at breaking down the adipose tissue and yielding a high ADSC recovery, these enzymes are expensive and can have detrimental effect on the ADSCs — including the risks of using xenogeneic components in clinical applications. This protocol details a method to isolate ADSCs from fresh lipoaspirate and abdominoplasty samples without enzymes. Briefly, this method relies on mechanical disassociation of any bulk tissue followed by an explant-type culture system. The ADSCs are permitted to migrate out of tissue and onto the tissue culture plate, after which the ADSCs can be cultured and expanded in vitro for any number of research and/or clinical applications.

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Keywords Adipose derived Mesenchymal Stem Cells

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