3D Modeling of Dendritic Spines with Synaptic Plasticity

Summary

The protocol develops a three-dimensional (3D) model of a dendritic segment with dendritic spines for modeling synaptic plasticity. The constructed mesh can be used for computational modeling of AMPA receptor trafficking in the long-term synaptic plasticity using the software program Blender with CellBlender and MCell.

Abstract

Computational modeling of diffusion and reaction of chemical species in a three-dimensional (3D) geometry is a fundamental method to understand the mechanisms of synaptic plasticity in dendritic spines. In this protocol, the detailed 3D structure of the dendrites and dendritic spines is modeled with meshes on the software Blender with CellBlender. The synaptic and extrasynaptic regions are defined on the mesh. Next, the synaptic receptor and synaptic anchor molecules are defined with their diffusion constants. Finally, the chemical reactions between synaptic receptors and synaptic anchors are included and the computational model is solved numerically with the software MCell. This method describes the spatiotemporal path of every single molecule in a 3D geometrical structure. Thus, it is very useful to study the trafficking of synaptic receptors in and out of the dendritic spines during the occurrence of synaptic plasticity. A limitation of this method is that the high number of molecules slows the speed of the simulations. Modeling of dendritic spines with this method allows the study of homosynaptic potentiation and depression within single spines and heterosynaptic plasticity between neighbor dendritic spines.

Introduction

Synaptic plasticity has been associated with learning and memory¹. Synaptic plasticity, such as long-term potentiation (LTP) and long-term depression (LTD), is associated respectively with the insertion and removal of AMPA receptors (AMPARs) in and out of the synaptic membrane². The AMPAR synapses are located on top of the small volume structures called dendritic spines³. Each spine contains a protein dense region in the postsynaptic membrane called the postsynaptic density (PSD). Anchor proteins at the PSD trap AMPARs in the synaptic region. There are few copies of AMPARs within a single synapse and the trafficking and reaction of AMPARs with other species in dendritic spines is a stochastic process^2,4. There are several compartmental models of synaptic receptor trafficking at dendritic spines^5,6,7,8. However, there is a lack of stochastic computational models of the trafficking of AMPARs associated with synaptic plasticity at the 3D structures of the dendrites and their dendritic spines.

Computational modeling is a useful tool to investigate the mechanisms underlying the dynamics of complex systems such as the reaction-diffusion of AMPARs in dendritic spines during the occurrence of synaptic plasticity^9,10,11,12. The model can be used to visualize complex scenarios, varying sensitive parameters and making important predictions in scientific conditions involving many variables that are difficult or impossible to control experimental^12,13. Defining the level of detail of a computational model is a fundamental step in obtaining accurate information about the modeled phenomenon. An ideal computational model is a delicate balance between complexity and simplicity to capture the essential characteristics of the natural phenomena without being computationally prohibitive. Computational models that are too detailed can be expensive to compute. On the other hand, systems that are poorly detailed can lack the fundamental components that are essential to capture the dynamics of the phenomenon. Although 3D modeling of dendritic spines is computationally more expensive than 2D and 1D, there are conditions, such as in complex systems with many nonlinear variables reacting and diffusing in time and 3D space, for which modeling at a 3D level is essential to obtain insights about the functioning of the system. Further, the complexity can be reduced carefully to preserve the essential characteristics of a lower-dimensional model.

In a stochastic system with few copies of a given species within a small volume, the average dynamics of the system deviates from the average dynamics of a large population. In this case, the stochastic computational modeling of reaction-diffusing particles is required. This work introduces a method for stochastic modeling reaction-diffusion of a few copies of AMPARs in 3D dendritic spines. The purpose of this method is to develop a 3D computational model of a dendritic segment with dendritic spines and their synapses for modeling synaptic plasticity.

The method uses the software MCell to solve the model numerically, Blender for constructing 3D meshes, and CellBlender to create and visualize the MCell simulations, including the spatiotemporal reaction-diffusion of molecules in 3D meshes^14,15,16. Blender is a suite for the creation of meshes and CellBlender is an add-on for the base software Blender. MCell is a Monte Carlo simulator for the reaction-diffusion of single molecules¹⁷.

The rationale behind the use of this method consists of modeling synaptic plasticity to achieve a better understanding of this phenomenon in the microphysiological environment of the dendritic spines¹⁴. Particularly, this method allows the simulation of homosynaptic potentiation, homosynaptic depression, and heterosynaptic plasticity between dendritic spines¹⁴.

The features of this method include modeling the 3D geometric structure of the dendrite and its synapses, the diffusion by random walk, and the chemical reactions of the molecules involved with synaptic plasticity. This method provides the advantage of creating rich environments to test hypotheses and make predictions about the functioning of a complex nonlinear system with a large number of variables. In addition, this method can be applied not only for studying synaptic plasticity but also for studying stochastic reaction-diffusion of molecules in 3D mesh structures in general.

Alternatively, 3D meshes of dendritic structures can be constructed directly in Blender from electron microscope serial reconstructions¹⁸. Although meshes based on serial reconstructions provide 3D structures, access to the experimental data is not always available. Thus, the construction of meshes adapted from basic geometric structures, as described in the present protocol, provides flexibility to develop customized dendritic segments with dendritic spines.

Another alternative computational method is the bulk simulation of well-mixed reactions in a regular volume^{9,10,11,19,20,21,22}. The bulk simulations are very efficient in solving the reactions of many species within a single well-mixed volume²³, but the bulk approach is extremely slow to solve the reaction-diffusion of molecules within many well-mixed voxels in a high-resolution 3D mesh. On the other hand, the present method using MCell simulations of reaction-diffusion of individual particles works efficiently in high-resolution 3D meshes¹⁵.

Before using this method, one should ask whether the phenomenon studied requires a stochastic reaction-diffusion approach in a 3D mesh. If the phenomenon has few copies (less than 1,000) of at least one of the reacting species diffusing in a complex geometric structure with small volume compartments such as dendritic spines, then stochastic modeling of reaction-diffusion in 3D meshes is appropriate for the application.

There are several steps required to construct a 3D computational model of a dendritic segment containing dendritic spines with synaptic plasticity. The main steps are the installation of the proper software for the construction of the model, the construction of a single dendritic spine to be used as a template to create multiple spines, and the creation of a dendritic segment that is connected with multiple dendritic spines. The step for modeling synaptic plasticity consists of inserting anchors at the PSD region and AMPARs in the dendritic segment and dendritic spines. Then, kinetic reactions between the anchors located at the PSD and AMPARs are defined to produce complexed anchor-AMPAR species that trap the AMPARs at the synaptic region. Respectively, the increase and decrease of the affinity between the anchors and the synaptic AMPARs create the process of LTP and LTD.

Protocol

NOTE: Please see the Supplementary file 1 for the glossary of terms used in this protocol.

1. Install Blender, CellBlender, and MCell

NOTE: This protocol requires installation of MCell, Blender, and Cell Blender.

1. Download and install the software on the MCell homepage (https://mcell.org/tutorials_iframe.html). Go to downloads on the top of the page and then follow the step-by-step instructions to download and install the software in the environment of choice (e.g., Linux, Mac OSX, or Windows).
   NOTE: All computational models and simulations described in this protocol were tested on a CellBlender 1.1 bundle that includes Blender 2.78 with MCell 3.4 and CellBlender 1.1. It worked also on Blender 2.79b. All these software programs are open access and do not require reprint permission to be used. The instructions for the construction and simulation of the model can change slightly from one version to another. Parts of this protocol have been adapted from Czech et al.¹⁶.

2. Create a single dendritic spine

NOTE: This procedure creates a mesh of a single dendritic spine with a spine head and a spine neck using a modified sphere.

1. Set up Blender 3D view at the Main Panel.
   1. Open Blender with CellBlender already installed. Press 5 on the keypad to change from Perspective to Orthogonal view and press 1 to change to front view. The perspective view has depth, but this is not needed now. Changing from Perspective to Orthogonal view allows better visualization of the mesh. Press Shift+C to center the cursor (Figure 1A).
2. Create the spine head.
   1. Press Shift+A to open the mesh palette. Select the Mesh and then select UV Sphere. A UV sphere is a mesh mapped to the 3D surface of a sphere. The UV sphere represents the spherical head of a mushroom dendritic spine. The software assumes the units of the UV sphere are micrometers.
   2. Change the parameters on the Add UV Sphere panel. Change Size to 0.25 and Rings to 32 (Figure 1B). Press + or – on the keypad to respectively zoom in and zoom out of the visualization of the mesh. Alternatively, use the scroll button in the mouse to zoom in and out (Figure 1C).
      NOTE: The parameter size scales the size of the original sphere and the parameter rings define the resolution of the mesh.
3. Make the top of the head flat.
   1. Press Tab to switch Blender from the Object Mode, the standard object interaction mode, to Edit Mode. Work in Edit Mode for modifying the components of an existing mesh.
   2. Once the created mesh has been automatically selected, press a to deselect the created mesh. Press z to make the mesh transparent, which helps in visualizing the parts that will be edited. Zoom in on the mesh. Press b to select the top ¾ of the sphere with the mouse (Figure 2A). Press delete, select vertices, and enter to remove the vertices (Figure 2B).
   3. Press b and select the top. Press e, s, 0, and enter to seal the top with the vertices still selected. Move the blue arrow down to align to the top of the spine head (Figure 2C). Press z to change to solid view (Figure 3A). Press 7 to change to top view.
      NOTE: The top of the sphere is made flat to model the PSD region of the spine head.
4. To increase the mesh resolution at the top of the spine first select Tool and Knife. Cut a circle with the knife around the center of the top (Figure 3B). Select Tool and Loop Cut and Slide. Repeat this step four times to create four concentric circles around the center of the top (Figure 3C).
   NOTE: The concentric circles are used to add new voxels that will increase the resolution of the PSD.
5. Create the spine neck.
   1. Press a to deselect the mesh. Press 1 to change to the front view. Press z to make the mesh transparent. Press b and then select the bottom of the mesh (Figure 4A). Press delete and vertices (Figure 4B). Press b and select the bottom of the mesh (Figure 4C). Press e and z, -0.45 to create an extrusion (Figure 4D).
      NOTE: This creates an extrusion to the z axis position at -0.45 μm. Press a to deselect the whole mesh.
   2. Press b and select the bottom of the neck. Press e, s, and 0 to seal the bottom (Figure 4E). Press a to select the whole mesh.
6. Make the mesh compatible with MCell.
   1. Press Crtl+T to triangulate the mesh. The mesh is transformed into a set of interconnected triangles. This is a required procedure to make the mesh compatible with MCell. Select Tool and Remove Doubles. Use the Remove Doubles tools to remove duplicated vertices, if any, that have the same coordinates or are very close to each other, to make the mesh compatible with MCell.
      NOTE: Double superposed vertices may have been accidentally created during the process of mesh creation and editing.
   2. Select Model Objects on the CellBlender panel. Change the name of the Active Object to spine and press + to create the object spine. On the CellBlender panel, select Mesh Analysis and then click on Analyze Mesh (Figure 4F). This procedure will analyze the properties of the created mesh, including the number of vertices, edges, faces, surface area, volume, and mesh topology.
      NOTE: The analysis will print the information in the Mesh Analysis Panel and it should be Watertight, Manifold, and Outward-Facing Normals. This step is required to ensure that the mesh will work on MCell. Otherwise, a step was probably missed. In this case, delete the mesh and start from step 2.1 again.
   3. Press z to visualize the solid view of the spine. Press File and Save to have a copy of your blender file with the spine on the disk.
      NOTE: The dimensions (i.e., length, diameter, size) of the meshes are in micrometers. See the glossary for the meaning of each keyboard shortcut.

3. Creating a dendrite with multiple spines

1. Generate a spine as described previously in sections 2.1–2.6. Press a to deselect the spine. Type Shift+C to center the cursor.
2. Create a dendrite. Press Shift+A to open the mesh palette. Select Mesh and then Cylinder. Change the parameters on the Add Cylinder menu: Radius = 0.3 μm, Depth = 2 μm. Press Enter.
   NOTE: The parameters radius and depth are defined according to the geometric characteristics of the dendrite.
3. Insert a spine in the dendrite.
   1. Press r and type 90 to rotate the cylinder 90° (Figure 5A). Use the blue arrow to drag the cylinder down to the bottom of the spine. Press 3 on the keypad to have a front view of the cylinder.
   2. Press z to make the mesh transparent. Use the mouse to move the blue normal arrow of the cylinder downwards to move the base of the spine to the interior of the cylinder (Figure 5B). Press a to deselect all objects.
   3. Use the right button of the mouse to select the dendrite (Figure 5C). Select Modifier on Blender panel (Figure 5D), select Add Modifier. Then select Boolean, select Operation Union, and select Object spine. Press Apply to create a joint mesh of the dendrite and the spine (Figure 5E). This operation creates a new mesh merging two meshes into a single one.
      NOTE: The new mesh will be the combined dendrite and spine. The isolated dendrite disappears when the different meshes are combined, but the isolated spine mesh remains overlapped with the new mesh and is used to generate multiple copies of the same spine. Delete all the isolated spines after finishing the mesh. It is critical to have a complete overlap between the spine neck and the dendrite, otherwise, the mesh will not be watertight.
4. Set the dendrite object into the CellBlender environment.
   1. Press a to deselect the meshes. Right-click in the dendrite with the mouse to select the dendrite only. Select CellBlender, Model Objects, and change Active Object to Dendrite and press + to create the Dendrite object.
5. Insert new spines in the dendrite.
   1. Press 1 to change to the side view of the cylinder. Use the mouse to select the mesh of the isolated spine. To insert more spines, follow step 3.3, changing the position and the angle to insert each one to obtain a physiological distribution.
6. Make the mesh compatible with MCell. To do so, press Tab to go to edit mode. Press a to select the whole mesh. Press Crtl+T to triangulate the mesh. Select Tool on the Blender panel and select Remove Doubles.
7. Stylize the meshes.
   1. Smooth the mesh. Press Tab to change to object mode. Select Tool on the Blender panel and select Smooth. Select CellBlender, Model Objects, and select Add a Material.
   2. Make the mesh transparent by selecting Object Transparent and Material Transparent. Change alpha to 0.5 and enter to make the mesh partially transparent. Press z to change to solid view.
8. Confirm whether the mesh is still compatible with MCell. To do so, select Mesh Analysis on the CellBlender panel to make sure that the mesh is still watertight, manifold mesh, and outward-facing normal.
9. Save the blender file as dendrite_with_spines.blend.

4. Define surface regions

NOTE: This procedure creates the surface regions of the mesh that later will be used to set up how the regions interact with the molecules.

1. Open the file dendrite_with_spines in the Blender environment. To do so, select File, Open, dendrite_with_spines.blend, and Open Blender File.
2. Prepare the mesh for defining the surface regions. To do so, press Tab to change to edit mode. Press z to change to transparent view (Viewport shading, wireframe). Press a to select the whole mesh of the dendrite with spines. Select Model Objects. Select Dendrite. Press t to hide the CellBlender panel and better visualize the whole mesh in the main panel.
   1. Use + and – on the keypad to zoom in and out or scroll with the mouse. This is required for better visualization of the top of the spines to select and define the surface regions. Press a to deselect the object. Press Tab to change to Edit Mode. Press t to show the CellBlender panel again.
3. Define the PSD surface region. To do so, press b and select the top of a dendritic spine with the mouse (Figure 6A,6B). Press + on Defined Surface Regions. Change the Region Name to PSD1 and click Assign (Figure 6C). Press a to deselect the object.
4. Define the extrasynaptic surface region. To do so, press b and select the region around the top of the dendritic spine with the mouse (Figure 6D). Repeat step 4.3 for the Region Name to Extra_syn1. Repeat the step 4.3 for the other spines to define the other regions of the mesh (PSD2, PSD3, PSD4, Extra_syn2, Extra_syn3, and Extra_syn4) (Figure 6F). Press a to deselect the object.
5. Define the surface regions of the ends of the dendrite. To do so, press b and select the left end of the dendrite. Change the Region Name to Left_end and click Assign. Press a to deselect the object. Press b and select the right end of the dendrite (Figure 6E). Change the Region Name to Right_end and click Assign.
   NOTE: Move the mesh to find the best position to select each Defined Region.

5. Create molecules

1. Create AMPARs. To do so, select Molecules on the CellBlender Panel. Select + on Defined Molecules to insert a new molecule and change Name to AMPAR. Change Molecule Type to Surface Molecule and Diffusion Constant to 0.05e-8 cm²/s¹⁴ to define the diffusion constant of AMPARs in the membrane (Figure 7A).
2. Create Anchors. To do so, select Molecules on the CellBlender Panel. Select + on Defined Molecules to insert a new molecule and change Name to anchor. Change Molecule Type to Surface Molecule and change Diffusion Constant to 0.001e-8 cm²/s¹⁴ to define the diffusion constant of anchors in the membrane (Figure 7A).
3. To create Anchors Bound to AMPARs, select Molecules on the CellBlender Panel. Select + on Defined Molecules to insert a new molecule. Change Name to anchor_AMPAR. Change Molecule Type to Surface Molecule. Change Diffusion Constant to 0.001e-8 cm²/s¹⁴.
4. Create the anchor_LTP and anchor_AMPAR_LTP. To do so, repeat step 5.2. Name the molecule anchor_LTP. Repeat step 5.3. Name the molecule anchor_AMPAR_LTP.
   NOTE: The anchor_LTP has a high affinity for AMPAR; thus, AMPARs increase in the synaptic regions.
5. Create the anchor_LTD and anchor_AMPAR_LTD. To create an anchor_LTD, repeat step 5.2. Name the molecule anchor_LTD. Repeat step 5.3. Name the molecule anchor_AMPAR_LTD.
   NOTE: The anchor_LTD has a low affinity for AMPAR; thus, AMPARs decrease in the synaptic region.

6. Define surface classes

NOTE: This procedure defines the classes with the properties that are associated with the surface regions. The extrasynaptic regions reflect the free anchors and anchors bound to AMPAR. The lateral ends of the dendrite reflect all the molecules.

1. Define the properties of the extrasynaptic regions.
   1. Press Tab to change to object mode. Select Surface Classes on the CellBlender Panel. Press + on the Surface Class to define a new surface class.
   2. Make the extrasynaptic region reflect the AMPAR bound to the anchor molecules.
      NOTE: This procedure will trap the anchors and everything bound to them within the synaptic region.
      1. Change Surface Class Name to reflective_extra_syn. Press + on reflective_extra_syn Properties to associate it with a molecule. Select Molecules | Single Molecule. Select anchor_AMPAR. Select Orientation = Ignore. Select Type = Reflective to make the region show the anchor_AMPAR molecules.
      2. Repeat step 6.1.2.1 for anchor_AMPAR_LTP and anchor_AMPAR_LTD.
   3. Make the extrasynaptic region reflect the anchors.
      1. Press + on reflective_extra_syn Properties to associate it with a molecule. Select Molecules | Single Molecule. Select anchor. Select Orientation = Ignore. Select Type = Reflective to make the region reflect the anchor molecules.
      2. Repeat step 6.1.3.1 for anchor_LTP and anchor_LTD.
2. Define the properties of the dendrite ends. To do so, press + on Surface Class to define a new surface class. Change Surface Class Name to reflective_ends. Press + on Properties to associate it with a molecule. Select Molecules | All Surface Molecules. Select Orientation | Ignore. Select Type | Reflective to make it reflect all surface molecules.

7. Assign the created classes to each surface region

NOTE: This step assigns the surface classes to the surface regions.

1. Assign the properties of the ends of the dendrite.
   1. Press + to assign a surface class with a region. Select reflective_ends for Surface Class Name (Figure 7C). Select Dendrite for Object Name. Select Specified Region for Region Selection. Select Left_end for Region Name.
   2. Repeat step 7.1.1 for the Right_end (Figure 7D).
2. Assign the properties of the extrasynaptic regions.
   1. Press + to assign a surface class with a region. Select reflective_extra_syn for Surface Class Name. Select Dendrite for Object Name. Select Specified Region for Region Selection. Select Extra_syn1 for Region Name.
   2. Repeat step 7.2.1 for Extra_syn2, Extra_syn3, and Extra_syn4.

8. Place molecules on the mesh

NOTE: This step places the AMPARs, anchors, and AMPAR bound to anchors on the mesh.

1. To place AMPAR Molecules on the Mesh, select Molecule Placement on the CellBlender Panel. Press + on the Release/Placement Sites to create a new release site. Change Site Name to relAMPAR (Figure 7B). Select Molecule = AMPAR. Object/Region = Dendrite[ALL]-(Dendrite[Left_end]+Dendrite[Right_end]). Quantity to Release = 1,000.
2. Place anchor molecules on the Mesh.
   1. Select Molecule Placement on the CellBlender Panel. Press + on the Release/Placement Sites to create a new release site. Change Site Name to rel_anchor_PSD1. Select Molecule anchor. Object/Region = Dendrite[PSD1]. Quantity to Release = 200.
   2. Repeat step 8.2.1 for PSD2, PSD3, and PSD4.
3. Place anchor_LTP Molecules on the Mesh. To do so, select Molecule Placement on the CellBlender Panel. Press + on the Release/Placement Sites to create a new release site. Change Site Name to rel_anchor_LTP_PSD1. Select Molecule = anchor_LTP. Object/Region = Dendrite[PSD1]. Quantity to Release = 0.
   NOTE: anchor_LTP is an anchor with high binding affinity for AMPARs.
4. Place anchor_LTD Molecules on the Mesh by repeating step 8.3 for anchor_LTD.
   NOTE: anchor_LTD is an anchor with low binding affinity for AMPARs.

9. Create the chemical reactions

1. Creating the reaction between anchor and AMPARs.
   1. Select Reactions (Figure 7D) to create the reactions. Press + to include a new reaction. Reactants = anchor’ + AMPAR’. Reaction Type = <->. This defines a bidirectional reaction. Products = anchor_AMPAR’. Forward Rate = 0.03. Backward Rate = 0.05.
2. Create the reaction between ANCHOR_LTP and AMPARs. To do so, repeat step 9.1, but replace anchor with anchor_LTP, and use a Backward Rate = 0.005 to increase the affinity between the reactants.
3. Create the reaction between anchor_LTD and AMPARs and save the file. To do so, repeat step 9.2, but replace anchor with anchor_LTD, and use a Backward Rate = 0.5 to decrease the affinity between the reactants. Then Save the file.

10. Plot the output of the model

1. Plot anchors bound to AMPARs at the PSD1 during the basal condition. To do so, select Plot Output Settings. Press + to define the molecules. Select anchor_AMPAR on Molecule. Select dendrite on Object. Select PSD1 on Region. Repeat step 10.1 for all PSD regions.
   NOTE: It is useful to observe the basal number of trapped AMPARs to the PSD of each dendritic spine. The number of anchors bound to AMPARs can increase or decrease in comparison to the basal conditions during LTP and LTD.
2. Plot anchors bound to AMPARs at the PSD1 during LTP. Do this by repeating step 10.1. Replace anchor_AMPAR with anchor_AMPAR_LTP, then plot anchors bound to AMPARs at the PSD1 during LTD and finally repeat step 10.1, but replace anchor_AMPAR_LTP with anchor_AMPAR_LTD.

11. Run the simulations

1. To run the basal condition, select Run Simulation. Select Iterations = 30,000. Set Time Step = 1e-3 s. Press Export & Run. Wait until the simulation ends. It may take from minutes to hours.
   NOTE: In the basal condition, there is no release of anchor_LTP and rel_anchor_LTD molecules. Regarding the parameters of the simulation, the number of iterations needs to be long enough to be able to observe the diffusion of AMPARs from the dendrites and their anchoring at PSD. Small time-steps are more precise but slower to complete the simulation.
2. Select Reload Visualization Data. Select play animation to visualize the spatiotemporal results (Figure 8). Select Plot Output Settings. Press Plot.
   NOTE: The graphs generated by CellBlender are isolated time series of the selected chemical species. Third-party programs can be used to import the data saved from multiple simulations to create overlaid plots of several conditions (e.g., basal, LTP, LTD; see Figure 8).
3. Run the homosynaptic potentiation condition (i.e., LTP; see Figure 8). To do so, select Molecule Placement on the CellBlender Panel. Select rel_anchor_LTP_PSD1 on the Release/Placement Sites.
4. Change quantity to release = 200. Select rel_anchor_LTD_PSD1 on the Release/Placement Sites. Change quantity to release = 0. Select rel_anchor _PSD1 on the Release/Placement Sites. Change quantity to release = 0. Repeat steps 11.1–11.2.
5. Run the homosynaptic depression condition (i.e., LTD; see Figure 8). To do so, Release 200 rel_anchor_LTD_PSD1 instead of rel_ANCHOR_LTP_PSD1. Set rel_anchor and rel_anchor_LTP_PSD1 to zero. Repeat steps 11.1–11.2.

Results

These results provide the steps for the construction of a 3D mesh that simulates a dendritic spine with a spine head and spine neck (Figure 1 to Figure 4). In addition, multiple dendritic spines can be inserted in a single dendritic segment (Figure 5) to study heterosynaptic plasticity of AMPARs¹⁴. The PSD on the top of the spine head (Figure 6) is the place where synaptic anchor...

Discussion

This article presents a method for the construction of 3D meshes for modeling reaction-diffusion synaptic plasticity processes in a dendritic segment with dendritic spines. The developed model contains a dendritic segment with few dendritic spines. The lateral diffusion and reaction of AMPARs with synaptic anchors allow the simulation of the basal dynamics. The critical steps in the protocol are cutting the sphere for the creation of the top of the spine head (Figure 1, ...

Materials

Name	Company	Catalog Number	Comments
Blender	Blender Foundation		https://www.blender.org/
CellBlender	University of Pittsburgh		https://mcell.org/
Mcell	University of Pittsburgh		https://mcell.org/