a-doxorubicin-induced-murine-model-of-dilated-cardiomyopathy-in-vivo

A Doxorubicin-Induced Murine Model of Dilated Cardiomyopathy In Vivo

Dilated cardiomyopathy (DCM) refers to a spectrum of heterogeneous myocardial disorders characterized by ventricular dilation and depressed cardiac ...

Clinical College of Nanjing Medical University

Previous studies showed that miR-124 had a protective role by reducing oxidant stress and preventing cell apoptosis and autophagy. However, its role in doxorubicin-induced cardiomyopathy was less known. In our study, we confirmed increased ROS and decreased expression of miR-124 in doxorubicin-treated heart tissues and primary cardiomyocytes. The oxidative stress and cell apoptosis were alleviated by overexpressing miR-124, characterized by decreased activity of MDA and increased activity of SOD. While inhibiting miR-124 generated opposed effects. Mechanistically, our bioinformatic prediction and luciferase assay confirmed that miR-124 inhibited the expression of p66Shc, a proapoptotic signaling pathway. Our results suggested that miR-124 was hopeful to become a therapeutic target in doxorubicin-related cardiomyopathy.

Tingting Hu

Department of Cardiology,
Nanjing Drum Tower Hospital,
Department of Cardiology, Nanjing Drum Tower Hospital

Clinical College of Nanjing Medical University

A Doxorubicin-Induced Murine Model of Dilated Cardiomyopathy In Vivo

Tingting Hu

.css-o250i3{font-size:18px;font-family:Open Sans,sans-serif;line-height:21.6px;}Department of Cardiology,Nanjing Drum Tower Hospital,Department of Cardiology, Nanjing Drum Tower Hospital

Clinical College of Nanjing Medical University

A Doxorubicin-Induced Murine Model of Dilated Cardiomyopathy In Vivo

Department of Cardiology,
Nanjing Drum Tower Hospital,
Department of Cardiology, Nanjing Drum Tower Hospital