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Hauenstein Neuroscience Center, Mercy Health Saint Mary’s

1 ARTICLES PUBLISHED IN JoVE

Neuroscience

Using Live-Cell Imaging to Measure the Effects of Pathological Proteins on Axonal Transport in Primary Hippocampal Neurons

Rebecca L. Mueller^1,2, Nicholas M. Kanaan^1,2,3, Benjamin Combs¹

¹Department of Translational Neuroscience, College of Human Medicine, Michigan State University, ²Neuroscience Program, Michigan State University, ³Hauenstein Neuroscience Center, Mercy Health Saint Mary’s

Here, we demonstrate how to combine transfection of primary hippocampal rodent neurons with live-cell confocal imaging to analyze pathological protein-induced effects on axonal transport and identify mechanistic pathways mediating these effects.