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Hauenstein Neuroscience Center, Mercy Health Saint Mary’s

1 ARTICLES PUBLISHED IN JoVE

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Neuroscience

Using Live-Cell Imaging to Measure the Effects of Pathological Proteins on Axonal Transport in Primary Hippocampal Neurons
Rebecca L. Mueller 1,2, Nicholas M. Kanaan 1,2,3, Benjamin Combs 1
1Department of Translational Neuroscience, College of Human Medicine, Michigan State University, 2Neuroscience Program, Michigan State University, 3Hauenstein Neuroscience Center, Mercy Health Saint Mary’s

Here, we demonstrate how to combine transfection of primary hippocampal rodent neurons with live-cell confocal imaging to analyze pathological protein-induced effects on axonal transport and identify mechanistic pathways mediating these effects.

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