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Italian National Research Council

3 ARTICLES PUBLISHED IN JoVE

Medicine

Francesca Rossi¹, Annalisa Canovetti², Alex Malandrini², Ivo Lenzetti², Roberto Pini¹, Luca Menabuoni²

¹Institute of Applied Physics, Italian National Research Council, ²U.O. Oculistica, Nuovo Ospedale Santo Stefano

An all laser procedure is proposed in the endothelial transplant. The surgical technique is based on the use of a femtosecond laser to prepare the donor tissue. Laser welding technique is then used to secure the donor endothelium in the correct position.

Bioengineering

Preparation and Photoacoustic Analysis of Cellular Vehicles Containing Gold Nanorods

Lucia Cavigli¹, Francesca Tatini¹, Claudia Borri², Fulvio Ratto¹, Sonia Centi¹, Alberto Cini³, Beatrice Lelli⁴, Paolo Matteini¹, Roberto Pini¹

¹Institute of Applied Physics, Italian National Research Council, ²Department of Experimental and Clinical Biomedical Sciences, University of Florence, Firenze, ³Department of Physics and Astronomy, University of Florence, Sesto Fiorentino, ⁴Department of Pharmacy and Biotechnology, University of Bologna

We show the preparation and address the feasibility of cellular vehicles containing gold nanorods for the photoacoustic imaging of cancer.

Biology

In Situ Immunofluorescent Staining of Autophagy in Muscle Stem Cells

Francesco Castagnetti^1,2, Elisabetta Fiacco³, Carol Imbriano⁴, Lucia Latella^1,2

¹Department of Medicine, Institute of Translational Pharmacology, Italian National Research Council, ²Epigenetics and Regenerative Medicine, IRCCS Fondazione Santa Lucia, ³Biological and Environmental Sciences and Engineering Division, King Abdullah University of Science and Technology (KAUST), ⁴Department of Life Sciences, University of Modena and Reggio Emilia

Active autophagy is associated with productive muscle regeneration, which is essential for Muscle Stem Cell (MuSC) activation. Here, we provide a protocol for the in situ detection of LC3, an autophagy marker in MyoD-positive MuSCs of muscle tissue sections from control and injured mice.