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Italian National Research Council

3 ARTICLES PUBLISHED IN JoVE

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Medicine

An “All-laser” Endothelial Transplant
Francesca Rossi 1, Annalisa Canovetti 2, Alex Malandrini 2, Ivo Lenzetti 2, Roberto Pini 1, Luca Menabuoni 2
1Institute of Applied Physics, Italian National Research Council, 2U.O. Oculistica, Nuovo Ospedale Santo Stefano

An all laser procedure is proposed in the endothelial transplant. The surgical technique is based on the use of a femtosecond laser to prepare the donor tissue. Laser welding technique is then used to secure the donor endothelium in the correct position.

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Bioengineering

Preparation and Photoacoustic Analysis of Cellular Vehicles Containing Gold Nanorods
Lucia Cavigli 1, Francesca Tatini 1, Claudia Borri 2, Fulvio Ratto 1, Sonia Centi 1, Alberto Cini 3, Beatrice Lelli 4, Paolo Matteini 1, Roberto Pini 1
1Institute of Applied Physics, Italian National Research Council, 2Department of Experimental and Clinical Biomedical Sciences, University of Florence, Firenze, 3Department of Physics and Astronomy, University of Florence, Sesto Fiorentino, 4Department of Pharmacy and Biotechnology, University of Bologna

We show the preparation and address the feasibility of cellular vehicles containing gold nanorods for the photoacoustic imaging of cancer.

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Biology

In Situ Immunofluorescent Staining of Autophagy in Muscle Stem Cells
Francesco Castagnetti 1,2, Elisabetta Fiacco 3, Carol Imbriano 4, Lucia Latella 1,2
1Department of Medicine, Institute of Translational Pharmacology, Italian National Research Council, 2Epigenetics and Regenerative Medicine, IRCCS Fondazione Santa Lucia, 3Biological and Environmental Sciences and Engineering Division, King Abdullah University of Science and Technology (KAUST), 4Department of Life Sciences, University of Modena and Reggio Emilia

Active autophagy is associated with productive muscle regeneration, which is essential for Muscle Stem Cell (MuSC) activation. Here, we provide a protocol for the in situ detection of LC3, an autophagy marker in MyoD-positive MuSCs of muscle tissue sections from control and injured mice.

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