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New York Institute of Technology

4 ARTICLES PUBLISHED IN JoVE

Medicine

Monitoring Tumor Metastases and Osteolytic Lesions with Bioluminescence and Micro CT Imaging

Ed Lim¹, Kshitij Modi¹, Anna Christensen¹, Jeff Meganck¹, Stephen Oldfield¹, Ning Zhang¹

¹Imaging Biology Research and Development, Caliper Life Sciences

An experimental mouse model of bone metastasis was established following intracardiac delivery of luciferase expressing mammary tumor cells. Tumor development and resulted osteolytic lesion were monitored longitudinally with bioluminescence and micro CT imaging.

Bioengineering

Construction of CRISPR Plasmids and Detection of Knockout Efficiency in Mammalian Cells through a Dual Luciferase Reporter System

Hegang Li^*1, Huaiyuan Qin^*1, Ning Zhang^*1, Jinshan Zhao¹, Jingjing Xin¹, Flor M. Perez-Campo², Huawei Liu¹

¹Qingdao Agricultural University, Qingdao, China, ²University of Cantabria, Santander y Torrelavega, Spain

Here, we present a protocol describing a streamlined method for the efficient generation of plasmids expressing both the CRISPR enzyme and associated single guide RNA (sgRNAs). Co-transfection of mammalian cells with this sgRNA/CRISPR vector and a dual luciferase reporter vector that examines double-strand break repair allows evaluation of knockout efficiency.

Genetics

2D-HELS MS Seq: A General LC-MS-Based Method for Direct and de novo Sequencing of RNA Mixtures with Different Nucleotide Modifications

Ning Zhang^1,2, Shundi Shi², Barney Yoo³, Xiaohong Yuan¹, Wenjia Li⁴, Shenglong Zhang¹

¹Department of Biological and Chemical Sciences, New York Institute of Technology, ²Department of Chemical Engineering, Columbia University, ³Department of Chemistry, Hunter College, City University of New York, ⁴Department of Computer Science, New York Institute of Technology

Here, we describe a detailed protocol for an LC-MS-based sequencing method that can be used as a direct method to sequence short RNA (<35 nt per run) without a cDNA intermediate, and as a general method to sequence different nucleotide modifications in a single study at single-base precision.

Medicine

Clinical Application of 24 G Cannula Needle and 3-0 Polypropylene Suture in Vas Deferens Exploration

Jin Wang^*1, Wenjia Li^*2, Yao He¹, Yuanbin Xia¹, Lin Sun¹, Youpeng Zhang¹, Zhaohui Zhu¹

¹Department of Urology, Union Hospital, Tongji Medical College, Huazhong University of Science and Technology, ²Department of Stomatology, Union Hospital, Tongji Medical College, Huazhong University of Science and Technology

This protocol presents the clinical application of a 24 G cannula and 3-0 polypropylene suture as a simple and effective method for the exploration of the vas deferens.