Pepperdine University
2 ARTICLES PUBLISHED IN JoVE
Neuroscience
Patch Clamp Recordings in Inner Ear Hair Cells Isolated from Zebrafish
Rachel Einarsson 1, Marshall Haden 1, Gabrielle DiCiolli 1, Andrea Lim 1, Kolina Mah-Ginn 1, Kathleen Aguilar 1, Lucy Yazejian 1, Bruce Yazejian 1
1Natural Science Division, Pepperdine University
The purpose of this video is to demonstrate procedures for obtaining healthy, intact hair cells from the inner ear organs of adult zebrafish and then using them for patch clamp studies aimed at characterizing the biophysical properties of their voltage-gated channels.
Neuroscience
Simultaneous Pre- and Post-synaptic Electrophysiological Recording from Xenopus Nerve-muscle Co-cultures
Bruce Yazejian 1, Rita M. Yazejian 1, Rachel Einarsson 2, Alan D. Grinnell 1
1Department of Physiology, David Geffen School of Medicine at UCLA, 2Natural Science Division, Pepperdine University
This video demonstrates the procedures used to grow primary cultures of embryonic Xenopus nerve and muscle cells and the usefulness of this preparation for making simultaneous pre- and post-synaptic patch clamp recordings.
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