Accedi

Pepperdine University

2 ARTICLES PUBLISHED IN JoVE

Neuroscience

Patch Clamp Recordings in Inner Ear Hair Cells Isolated from Zebrafish

Rachel Einarsson¹, Marshall Haden¹, Gabrielle DiCiolli¹, Andrea Lim¹, Kolina Mah-Ginn¹, Kathleen Aguilar¹, Lucy Yazejian¹, Bruce Yazejian¹

¹Natural Science Division, Pepperdine University

The purpose of this video is to demonstrate procedures for obtaining healthy, intact hair cells from the inner ear organs of adult zebrafish and then using them for patch clamp studies aimed at characterizing the biophysical properties of their voltage-gated channels.

Neuroscience

Simultaneous Pre- and Post-synaptic Electrophysiological Recording from Xenopus Nerve-muscle Co-cultures

Bruce Yazejian¹, Rita M. Yazejian¹, Rachel Einarsson², Alan D. Grinnell¹

¹Department of Physiology, David Geffen School of Medicine at UCLA, ²Natural Science Division, Pepperdine University

This video demonstrates the procedures used to grow primary cultures of embryonic Xenopus nerve and muscle cells and the usefulness of this preparation for making simultaneous pre- and post-synaptic patch clamp recordings.