Harvey Mudd College
2 ARTICLES PUBLISHED IN JoVE
Immunology and Infection
Detection of Trypanosoma brucei Variant Surface Glycoprotein Switching by Magnetic Activated Cell Sorting and Flow Cytometry
Danae Schulz 1,2, Monica R. Mugnier 1, Catherine E. Boothroyd 1,3, F. Nina Papavasiliou 1
1Laboratory of Lymphocyte Biology, Rockefeller University, 2Department of Biology, Harvey Mudd College, 3Masters School
African trypanosomes grown in vitro undergo antigenic variation at a low rate, such that populations are made up of parasites expressing a dominant variant surface glycoprotein (VSG) type and a small population of "switched" variants. This protocol describes a fast method for detecting and quantifying these populations.
Environment
Laser-Induced Fluorescence Emission (L.I.F.E.) as Novel Non-Invasive Tool for In-Situ Measurements of Biomarkers in Cryospheric Habitats
Klemens Weisleitner 1,2, Lars Hunger 3, Christoph Kohstall 4, Albert Frisch 5, Michael C. Storrie-Lombardi 6, Birgit Sattler 1,2
1Institute of Ecology, University of Innsbruck, 2Austrian Polar Research Institute, University of Vienna, 3BrainLinks-BrainTools, Bernstein Center Freiburg, 4Atom Science, Kasevich Lab, Stanford University, 5Institute of Experimental Physics, University of Innsbruck, 6Department of Physics, Extraterrestrial Vehicle Instruments Laboratory, Harvey Mudd College
Carbon fluxes in the cryosphere are hardly assessed yet but are crucial regarding climate change. Here we show a novel prototype device that captures the phototrophic potential in supraglacial environments based on laser-induced fluorescence emission (L.I.F.E.) technology offering high spectral and spatial resolution data under in situ conditions.
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