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The City University of New York

3 ARTICLES PUBLISHED IN JoVE

Immunology and Infection

An Introduction to Parasitic Wasps of Drosophila and the Antiparasite Immune Response

Chiyedza Small^*1,2, Indira Paddibhatla^*1,2, Roma Rajwani¹, Shubha Govind^1,2

¹Biology Department, The City College of New York, CUNY, ²The Graduate Center, The City University of New York

Parasitoid (parasitic) wasps constitute a major class of natural enemies of many insects including Drosophila melanogaster. We will introduce the techniques to propagate these parasites in Drosophila spp. and demonstrate how to analyze their effects on immune tissues of Drosophila larvae.

Biology

Sample Preparation for Metabolic Profiling using MALDI Mass Spectrometry Imaging

Kelly Veerasammy^*1,2, Yuki X. Chen^*1,2, Sami Sauma^*1, Mathilde Pruvost¹, David K. Dansu¹, Tenzin Choetso^1,2, Tiffany Zhong³, Damien Marechal¹, Patrizia Casaccia¹, Rinat Abzalimov^4,5, Ye He^1,5

¹The Graduate Center - Advanced Science Research Center, Neuroscience Initiative, The City University of New York, ²The City College of New York, CUNY, ³The Bronx High School of Science, ⁴The Graduate Center - Advanced Science Research Center, Structural Biology Initiative, The City University of New York, ⁵The Graduate Center - Advanced Science Research Center, MALDI MS Imaging Joint Core Facility, The City University of New York

The goal of this protocol is to provide detailed guidance on the sample preparation when planning for experiments using MALDI MSI to maximize metabolic and molecular detection in biological samples.

Biology

Sample Preparation for Rapid Lipid Analysis in Drosophila Brain Using Matrix-Assisted Laser Desorption/Ionization Mass Spectrometry Imaging

Yuki X. Chen^*1,5,6, Kelly Veerasammy^*1,2, Jun Yin^*3, Tenzin Choetso^1,4, Tiffany Zhong⁷, Muniyat A. Choudhury^1,4,5, Cory Weng^1,4,5, Ethan Xu⁸, Mayan A. Hein^9,10, Rinat Abzalimov², Ye He¹

¹Advanced Science Research Center, Neuroscience Initiative, the City University of New York, Graduate Center New York, ²Advanced Science Research Center, Structural Biology Initiative, the City University of New York, Graduate Center, ³National Institute of Neurological Disorders and Stroke, National Institutes of Health, ⁴The City College of New York, CUNY, ⁵Macaulay Honors College, CUNY, ⁶Graduate School of Public Health and Health Policy, The City University of New York, ⁷Princeton University, ⁸Ardrey Kell High School, ⁹The Borough of Manhattan Community College, CUNY, ¹⁰Gallatin School of Individualized Study, New York University

The aim of this protocol is to provide detailed guidance on the proper sample preparation for lipid and metabolite analysis in small tissues, such as the Drosophila brain, using matrix-assisted laser desorption/ionization (MALDI) mass spectrometry imaging.