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Michael E. DeBakey VA Medical Center

5 ARTICLES PUBLISHED IN JoVE

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Biology

A Reversible, Non-invasive Method for Airway Resistance Measurements and Bronchoalveolar Lavage Fluid Sampling in Mice
Sumanth Polikepahad 1, Wade T. Barranco 1, Paul Porter 1, Bruce Anderson 2, Farrah Kheradmand 1,3, David B. Corry 1,3
1Department of Medicine, Baylor College of Medicine (BCM), 2Millenium Premier Group, 3Department of Immunology, Baylor College of Medicine (BCM)

Repeated measurements of rodent respiratory physiology and sampling of airway inflammatory cells are desirable, but generally not feasible. Here we describe a repeatable method for orally intubating mice that permits repeated measurements of airway hyperreactivity and sampling of airway inflammatory cells.

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Immunology and Infection

piggyBac Transposon System Modification of Primary Human T Cells
Sunandan Saha 1,2, Yozo Nakazawa 3, Leslie E. Huye 4,5, Joseph E. Doherty 2,6, Daniel L. Galvan 2, Cliona M. Rooney 4,5,7, Matthew H. Wilson 1,2,4,8
1Program in Translational Biology and Molecular Medicine, Baylor College of Medicine , 2Department of Medicine, Division of Nephrology, Baylor College of Medicine , 3Department of Immunology and Pathology, Shinshu University School of Medicine, 4Center for Cell and Gene Therapy, Baylor College of Medicine , 5Department of Pediatrics, Baylor College of Medicine , 6Program in Cell and Molecular Biology, Baylor College of Medicine , 7Department of Molecular Virology and Microbiology, Baylor College of Medicine , 8Michael E. DeBakey VA Medical Center

We describe a method to genetically modify primary human T cells with a transgene using the non-viral piggyBac transposon system. T cells modified to using the piggyBac transposon system exhibit stable transgene expression.

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Medicine

Network Analysis of the Default Mode Network Using Functional Connectivity MRI in Temporal Lobe Epilepsy
Zulfi Haneef 1,2, Agatha Lenartowicz 3, Hsiang J. Yeh 4, Jerome Engel Jr. 4, John M. Stern 4
1Department of Neurology, Baylor College of Medicine, 2Neurology Care Line, Michael E. DeBakey VA Medical Center, 3Semel Institute for Neuroscience and Human Behavior, University of California, Los Angeles, 4Department of Neurology, University of California, Los Angeles

The Default Mode Network (DMN) in Temporal Lobe Epilepsy (TLE) is analyzed in the resting state of the brain using seed-based functional connectivity MRI (fcMRI).

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Biology

Hydrodynamic Renal Pelvis Injection for Non-viral Expression of Proteins in the Kidney
Lauren E. Woodard 1,2,3, Richard C. Welch 2, Felisha M. Williams 2, Wentian Luo 2, Jizhong Cheng 3, Matthew H. Wilson 1,2,3
1Department of Veterans Affairs, Tennessee Valley Healthcare System, 2Departments of Medicine and Pharmacology, Vanderbilt University Medical Center, 3Department of Medicine, Baylor University College of Medicine

This protocol describes a method to inject plasmid DNA into the mouse kidney via the renal pelvis to produce transgene expression specifically in the kidney.

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Immunology and Infection

Cigarette Smoke Exposure in Mice using a Whole-Body Inhalation System
Daniel E. Morales-Mantilla 1,2, Xinyan Huang 3,7, Philip Erice 1,3, Paul Porter 4, Yun Zhang 1,5, Mary Figueroa 6, Joya Chandra 6, Katherine Y. King 2, Farrah Kheradmand 4,8, Antony Rodríguez 3,8
1Program in Immunology, Baylor College of Medicine, 2Department of Pediatrics, Section of Infectious Diseases, Baylor College of Medicine, 3Department of Medicine - Immunology Allergy and Rheumatology, Baylor College of Medicine, 4Department of Medicine, Pulmonary, Critical Care, Sleep Medicine, Baylor College of Medicine, 5Department of Pathology and Immunology, Baylor College of Medicine, 6Department of Pediatrics, Research and Department of Epigenetics and Molecular Carcinogenesis, The University of Texas MD Anderson Cancer Center, 7Division of Pulmonary and Critical Care Medicine, The First Affiliated Hospital of Sun Yat-sen University, 8Center for Translational Research on Inflammatory Diseases (CTRID), Michael E. DeBakey VA Medical Center

This protocol demonstrates the study of the pathophysiologic effects of cigarette smoke (CS) with a whole-body inhalation (WBI) exposure system (WBIS) built in-house. This system can expose animals to CS under controlled repeatable conditions for research of CS-mediated effects on lung emphysema and hematopoiesis.

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