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Michael E. DeBakey VA Medical Center

5 ARTICLES PUBLISHED IN JoVE

Biology

A Reversible, Non-invasive Method for Airway Resistance Measurements and Bronchoalveolar Lavage Fluid Sampling in Mice

Sumanth Polikepahad¹, Wade T. Barranco¹, Paul Porter¹, Bruce Anderson², Farrah Kheradmand^1,3, David B. Corry^1,3

¹Department of Medicine, Baylor College of Medicine (BCM), ²Millenium Premier Group, ³Department of Immunology, Baylor College of Medicine (BCM)

Repeated measurements of rodent respiratory physiology and sampling of airway inflammatory cells are desirable, but generally not feasible. Here we describe a repeatable method for orally intubating mice that permits repeated measurements of airway hyperreactivity and sampling of airway inflammatory cells.

Immunology and Infection

piggyBac Transposon System Modification of Primary Human T Cells

Sunandan Saha^1,2, Yozo Nakazawa³, Leslie E. Huye^4,5, Joseph E. Doherty^2,6, Daniel L. Galvan², Cliona M. Rooney^4,5,7, Matthew H. Wilson^1,2,4,8

¹Program in Translational Biology and Molecular Medicine, Baylor College of Medicine , ²Department of Medicine, Division of Nephrology, Baylor College of Medicine , ³Department of Immunology and Pathology, Shinshu University School of Medicine, ⁴Center for Cell and Gene Therapy, Baylor College of Medicine , ⁵Department of Pediatrics, Baylor College of Medicine , ⁶Program in Cell and Molecular Biology, Baylor College of Medicine , ⁷Department of Molecular Virology and Microbiology, Baylor College of Medicine , ⁸Michael E. DeBakey VA Medical Center

We describe a method to genetically modify primary human T cells with a transgene using the non-viral piggyBac transposon system. T cells modified to using the piggyBac transposon system exhibit stable transgene expression.

Medicine

Network Analysis of the Default Mode Network Using Functional Connectivity MRI in Temporal Lobe Epilepsy

Zulfi Haneef^1,2, Agatha Lenartowicz³, Hsiang J. Yeh⁴, Jerome Engel Jr.⁴, John M. Stern⁴

¹Department of Neurology, Baylor College of Medicine, ²Neurology Care Line, Michael E. DeBakey VA Medical Center, ³Semel Institute for Neuroscience and Human Behavior, University of California, Los Angeles, ⁴Department of Neurology, University of California, Los Angeles

The Default Mode Network (DMN) in Temporal Lobe Epilepsy (TLE) is analyzed in the resting state of the brain using seed-based functional connectivity MRI (fcMRI).

Biology

Hydrodynamic Renal Pelvis Injection for Non-viral Expression of Proteins in the Kidney

Lauren E. Woodard^1,2,3, Richard C. Welch², Felisha M. Williams², Wentian Luo², Jizhong Cheng³, Matthew H. Wilson^1,2,3

¹Department of Veterans Affairs, Tennessee Valley Healthcare System, ²Departments of Medicine and Pharmacology, Vanderbilt University Medical Center, ³Department of Medicine, Baylor University College of Medicine

This protocol describes a method to inject plasmid DNA into the mouse kidney via the renal pelvis to produce transgene expression specifically in the kidney.

Immunology and Infection

Cigarette Smoke Exposure in Mice using a Whole-Body Inhalation System

Daniel E. Morales-Mantilla^1,2, Xinyan Huang^3,7, Philip Erice^1,3, Paul Porter⁴, Yun Zhang^1,5, Mary Figueroa⁶, Joya Chandra⁶, Katherine Y. King², Farrah Kheradmand^4,8, Antony Rodríguez^3,8

¹Program in Immunology, Baylor College of Medicine, ²Department of Pediatrics, Section of Infectious Diseases, Baylor College of Medicine, ³Department of Medicine - Immunology Allergy and Rheumatology, Baylor College of Medicine, ⁴Department of Medicine, Pulmonary, Critical Care, Sleep Medicine, Baylor College of Medicine, ⁵Department of Pathology and Immunology, Baylor College of Medicine, ⁶Department of Pediatrics, Research and Department of Epigenetics and Molecular Carcinogenesis, The University of Texas MD Anderson Cancer Center, ⁷Division of Pulmonary and Critical Care Medicine, The First Affiliated Hospital of Sun Yat-sen University, ⁸Center for Translational Research on Inflammatory Diseases (CTRID), Michael E. DeBakey VA Medical Center

This protocol demonstrates the study of the pathophysiologic effects of cigarette smoke (CS) with a whole-body inhalation (WBI) exposure system (WBIS) built in-house. This system can expose animals to CS under controlled repeatable conditions for research of CS-mediated effects on lung emphysema and hematopoiesis.