Primer insan T hücrelerinin piggyBac Transposon Sistem Modifikasyon

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17.2K Views

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10:02 min

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November 5th, 2012

DOI :

10.3791/4235-v

November 5th, 2012

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Sunandan Saha¹^,², Yozo Nakazawa³, Leslie E. Huye⁴^,⁵, Joseph E. Doherty²^,⁶, Daniel L. Galvan², Cliona M. Rooney⁴^,⁵^,⁷, Matthew H. Wilson¹^,²^,⁴^,⁸

¹Program in Translational Biology and Molecular Medicine, Baylor College of Medicine , ²Department of Medicine, Division of Nephrology, Baylor College of Medicine , ³Department of Immunology and Pathology, Shinshu University School of Medicine, ⁴Center for Cell and Gene Therapy, Baylor College of Medicine , ⁵Department of Pediatrics, Baylor College of Medicine , ⁶Program in Cell and Molecular Biology, Baylor College of Medicine , ⁷Department of Molecular Virology and Microbiology, Baylor College of Medicine , ⁸Michael E. DeBakey VA Medical Center

Özet

Daha Fazla Video Keşfet

Bu videodaki bölümler

0:05

Title

1:39

Day 0: Isolation of PBMCs from Human Blood

3:23

Day 1: Coating Plates with anti-CD28 and anti-CD3 Antibody for Stimulating T Cells

3:59

Day 1: Nucleofection of Unstimulated T Cells

5:54

Day 2: Nonspecific Stimulation of T Cells

7:28

Day 7: Analysis of Gene Expression

8:05

Results: piggyBac-mediated Gene Modification of Human T Cells

9:19

Conclusion

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