University of Missouri School of Medicine
2 ARTICLES PUBLISHED IN JoVE
Biology
Single-Cell Multiplexed Fluorescence Imaging to Visualize Viral Nucleic Acids and Proteins and Monitor HIV, HTLV, HBV, HCV, Zika Virus, and Influenza Infection
Raven Shah *1, Shuiyun Lan *1, Maritza N. Puray-Chavez 2, Dandan Liu 2, Philip R. Tedbury 1, Stefan G. Sarafianos 1
1Laboratory of Biochemical Pharmacology, Department of Pediatrics, Emory University School of Medicine, 2Department of Molecular Microbiology & Immunology, University of Missouri School of Medicine
Presented here is a protocol for a fluorescence imaging approach, multiplex immunofluorescent cell-based detection of DNA, RNA, and protein (MICDDRP), a method capable of simultaneous fluorescence single-cell visualization of viral protein and nucleic acids of different type and strandedness. This approach can be applied to a diverse range of systems.
Neuroscience
Minimally Invasive Murine Laryngoscopy for Close-Up Imaging of Laryngeal Motion During Breathing and Swallowing
Teresa E. Lever 1,2, Ashley Kloepper 1, Lauren Welby 3, Megan Haney 4, Shakera Fudge 4, Chase Seiller 5, Sophia Kington 2, Brayton Ballenger 5, Nicole L. Nichols 2
1Department of Otolaryngology - Head and Neck Surgery, University of Missouri School of Medicine, 2Department of Biomedical Sciences, University of Missouri College of Veterinary Medicine, 3Division of Otolaryngology - Head and Neck Surgery, Texas A&M Health Science Center College of Medicine - Baylor Scott & White, 4Department of Veterinary Pathobiology, University of Missouri College of Veterinary Medicine, 5Department of Medicine, University of Missouri School of Medicine
This protocol describes a serial transoral laryngoscopy approach for mice and rats that permits close-up, unobstructed video imaging of the larynx during breathing and swallowing using an optimized anesthetic regimen and finely tuned endoscopic manipulation techniques.
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