Accedi

University of Missouri School of Medicine

2 ARTICLES PUBLISHED IN JoVE

Biology

Single-Cell Multiplexed Fluorescence Imaging to Visualize Viral Nucleic Acids and Proteins and Monitor HIV, HTLV, HBV, HCV, Zika Virus, and Influenza Infection

Raven Shah^*1, Shuiyun Lan^*1, Maritza N. Puray-Chavez², Dandan Liu², Philip R. Tedbury¹, Stefan G. Sarafianos¹

¹Laboratory of Biochemical Pharmacology, Department of Pediatrics, Emory University School of Medicine, ²Department of Molecular Microbiology & Immunology, University of Missouri School of Medicine

Presented here is a protocol for a fluorescence imaging approach, multiplex immunofluorescent cell-based detection of DNA, RNA, and protein (MICDDRP), a method capable of simultaneous fluorescence single-cell visualization of viral protein and nucleic acids of different type and strandedness. This approach can be applied to a diverse range of systems.

Neuroscience

Minimally Invasive Murine Laryngoscopy for Close-Up Imaging of Laryngeal Motion During Breathing and Swallowing

Teresa E. Lever^1,2, Ashley Kloepper¹, Lauren Welby³, Megan Haney⁴, Shakera Fudge⁴, Chase Seiller⁵, Sophia Kington², Brayton Ballenger⁵, Nicole L. Nichols²

¹Department of Otolaryngology - Head and Neck Surgery, University of Missouri School of Medicine, ²Department of Biomedical Sciences, University of Missouri College of Veterinary Medicine, ³Division of Otolaryngology - Head and Neck Surgery, Texas A&M Health Science Center College of Medicine - Baylor Scott & White, ⁴Department of Veterinary Pathobiology, University of Missouri College of Veterinary Medicine, ⁵Department of Medicine, University of Missouri School of Medicine

This protocol describes a serial transoral laryngoscopy approach for mice and rats that permits close-up, unobstructed video imaging of the larynx during breathing and swallowing using an optimized anesthetic regimen and finely tuned endoscopic manipulation techniques.