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Ospedale Policlinico di Milano

2 ARTICLES PUBLISHED IN JoVE

Biology

A Novel Method for the Culture and Polarized Stimulation of Human Intestinal Mucosa Explants

Katerina Tsilingiri¹, Angelica Sonzogni², Flavio Caprioli³, Maria Rescigno¹

¹Department of Experimental Oncology, European Institute of Oncology, ²Department of Pathology and Laboratory Medicine, European Institute of Oncology, ³U.O. Gastroenterologia 2, IRCCS Ca' Granda, Ospedale Policlinico di Milano

We introduce a novel method for the maintenance of human intestinal mucosa in culture and monitoring of the response to various types of stimuli over at least 24 hrs. With our method, the polarity of the tissue is maintained, allowing for a physiological stimulation via the apical route.

Immunology and Infection

Using Robotic Systems to Process and Embed Colonic Murine Samples for Histological Analyses

Fulvia Milena Cribiù^*1, Claudia Burrello^*2, Roberta Tacchi¹, Francesca Boggio¹, Dario Ricca¹, Flavio Caprioli^3,4, Stefano Ferrero¹, Federica Facciotti²

¹Pathology Unit, Fondazione IRCCS Cà Granda, Ospedale Maggiore Policlinico, ²Department of Experimental Oncology, European Institute of Oncology, ³Gastroenterology and Endoscopy Unit, Fondazione IRCCS Cà Granda, Ospedale Maggiore Policlinico, ⁴Department of Pathophysiology and Transplantation, Università degli Studi di Milano

Lack of standardization for murine tissue processing reduces the quality of murine histopathological analysis as compared to human specimens. Here, we present a protocol to perform histopathological examination of murine inflamed and uninflamed colonic tissues to show the feasibility of robotic systems routinely used for processing and embedding human samples.