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Zhengzhou University

5 ARTICLES PUBLISHED IN JoVE

Immunology and Infection

A Simple and Efficient Approach to Construct Mutant Vaccinia Virus Vectors

Ming Yuan^*1, Pengju Wang^*2,3, Louisa S. Chard¹, Nicholas R. Lemoine^1,2,3, Yaohe Wang^1,2,3

¹Center for Molecular Oncology, Barts Cancer Institute, Queen Mary University of London, ²Sino-British Research Centre for Molecular Oncology, National Center for International Research in Cell and Gene Therapy, Zhengzhou University, ³School of Basic Medical Sciences, Academy of Medical Sciences, Zhengzhou University

Vaccinia virus (VV) has been widely used in biomedical research and the improvement of human health. This article describes a simple, highly efficient method to edit the VV genome using a CRISPR-Cas9 system.

Bioengineering

Production of Genetically Engineered Golden Syrian Hamsters by Pronuclear Injection of the CRISPR/Cas9 Complex

Rong Li^*1, Jinxin Miao^*1,2, Zhiqiang Fan¹, SeokHwan Song³, Il-keun Kong^3,4, Yaohe Wang^2,5, Zhongde Wang¹

¹Department of Animal, Dairy, and Veterinary Sciences, Utah State University, ²National Centre for International Research in Cell and Gene Therapy, Sino-British Research Centre, School of Basic Medical Sciences, Zhengzhou University, ³Department of Animal Science Division of Applied Life Science (BK21 Plus), Gyeongsang National University, ⁴Institute of Agriculture and Life Science, Gyeongsang National University, ⁵Centre for Molecular Oncology, Barts Cancer Institute, Queen Mary University of London

Pronuclear (PN) injection of the clustered regularly interspaced short palindromic repeats (CRISPR) and CRISPR-associated protein-9 nuclease (CRISPR/Cas9) system is a highly efficient method for producing genetically engineered golden Syrian hamsters. Herein, we describe the detailed PN injection protocol for the production of gene knockout hamsters with the CRISPR/Cas9 system.

Bioengineering

Protocols of 3D Bioprinting of Gelatin Methacryloyl Hydrogel Based Bioinks

Mingjun Xie^1,2, Kang Yu^1,2, Yuan Sun^1,2, Lei Shao^1,2, Jing Nie^1,2, Qing Gao^1,2, Jingjiang Qiu³, Jianzhong Fu^1,2, Zichen Chen^1,2, Yong He^1,2

¹State Key Laboratory of Fluid Power and Mechatronic Systems, School of Mechanical Engineering, Zhejiang University, ²Key Laboratory of 3D Printing Process and Equipment of Zhejiang Province, School of Mechanical Engineering, Zhejiang University, ³School of Mechanics and Safety Engineering, Zhengzhou University

Presented here is a method for the 3D bioprinting of gelatin methacryloyl.

Immunology and Infection

In Vivo Augmentation of Gut-Homing Regulatory T Cell Induction

Hongzheng Bi^1,2, Samiksha Wasnik¹, David J. Baylink¹, Chenfan Liu^1,3,4, Xiaolei Tang^1,3

¹Division of Regenerative Medicine, Department of Medicine, Loma Linda University, ²Zhengzhou University, ³Department of Veterinary Biomedical Sciences, College of Veterinary Medicine, Long Island University, ⁴Jinan Infectious Disease Hospital, Shandong University

Here we present a protocol for in vivo augmentation of gut-homing regulatory T cell induction. In this protocol, dendritic cells are engineered to locally produce high concentrations of the active vitamin D (1,25-dihydroxyvitamin D or 1,25[OH]₂D) and the active vitamin A (retinoic acid or RA) de novo.

Immunology and Infection

Quantitative Polymerase Chain Reaction (qPCR)-Based Rapid Diagnosis of Helicobacter pylori Infection and Antibiotic Resistance

Liang Wang^*1,2,3, Jin-Xin Lai^*1, Yu-Ting Si^*1,4, Xu-Xia Cui^1,4, Zeeshan Umar^1,5, Xiao-Jun Ru¹, Xin-Yu Zhang¹, Zheng-Kang Li¹, Alfred Chin Yen Tay^5,6,7,8, Barry J. Marshall^5,6,7,8, Guang-Hua Li¹, Bing Gu¹

¹Laboratory Medicine, Guangdong Provincial People’s Hospital (Guangdong Academy of Medical Sciences), Southern Medical University, ²Division of Microbiology and Immunology, School of Biomedical Sciences, The University of Western Australia, ³Center for Precision Health, School of Medical and Health Sciences, Edith Cowan University, ⁴Medical Technology School of Xuzhou Medical University, ⁵Marshall Laboratory of Biomedical Engineering, School of Medicine, Shenzhen University, ⁶The Marshall Centre for Infectious Diseases Research and Training, The University of Western Australia, ⁷Marshall International Digestive Diseases Hospital, Zhengzhou University, ⁸Marshall Medical Research Center, Fifth Affiliated Hospital of Zhengzhou University

The protocol presents a noninvasive method for the rapid diagnosis of Helicobacter pylori stomach infections through the string test and determines its antibiotic resistance to clarithromycin and levofloxacin using quantitative polymerase chain reaction (qPCR).