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Tai'an City Central Hospital of Shandong Province

5 ARTICLES PUBLISHED IN JoVE

Neuroscience

Thinned-skull Cortical Window Technique for In Vivo Optical Coherence Tomography Imaging

Jenny I. Szu¹, Melissa M. Eberle², Carissa L. Reynolds², Mike S. Hsu¹, Yan Wang², Christian M. Oh², M. Shahidul Islam², B. Hyle Park², Devin K. Binder¹

¹Division of Biomedical Sciences, University of California, Riverside , ²Department of Bioengineering, University of California, Riverside

We present a method of creating a thinned-skull cortical window (TSCW) in a mouse model for in vivo OCT imaging of the cerebral cortex.

Medicine

Rat Model of Photochemically-Induced Posterior Ischemic Optic Neuropathy

Yan Wang^1,3,4, Dale P. Brown¹, Brant D. Watson², Jeffrey L. Goldberg^1,3

¹Bascom Palmer Eye Institute, University of Miami Miller School of Medicine, ²Departments of Neurology and Biomedical Engineering, University of Miami Miller School of Medicine, ³Shiley Eye Center, University of California, ⁴Department of Ophthalmology and Vision Science, Fudan University

The goal of this protocol is to photochemically induce ischemic injury to the posterior optic nerve in rat. This model is critical to studies of the pathophysiology of posterior ischemic optic neuropathy, and therapeutic approaches for this and other optic neuropathies, as well as of other CNS ischemic diseases.

JoVE Journal

Isolation and Respiratory Measurements of Mitochondria from Arabidopsis thaliana

Wenhui Lyu¹, Jennifer Selinski¹, Lu Li¹, David A. Day², Monika W. Murcha³, James Whelan¹, Yan Wang¹

¹ARC Centre of Excellence in Plant Energy Biology, Department of Animal, Plant and Soil Science, School of Life Science, La Trobe University, ²School of Biological Sciences, Flinders University, ³ARC Centre of Excellence in Plant Energy Biology, University of Western Australia

As mitochondria are only a small percentage of the plant cell, they need to be purified for a range of studies. Mitochondria can be isolated from a variety of plant organs by homogenization, followed by differential and density gradient centrifugation to obtain a highly purified mitochondrial fraction.

Medicine

Protocol and Guidelines for Point-of-Care Lung Ultrasound in Diagnosing Neonatal Pulmonary Diseases Based on International Expert Consensus

Jing Liu^1,2, Roberto Copetti³, Erich Sorantin⁴, Jovan Lovrenski⁵, Javier Rodriguez-Fanjul⁶, Dalibor Kurepa⁷, Xing Feng⁸, Luigi Cattaross⁹, Huayan Zhang^10,11, Misun Hwang¹², Tsu F. Yeh^13,14, Yisrael Lipener⁷, Abhay Lodha¹⁵, Jia-Qin Wang¹⁶, Hai-Ying Cao^2,17, Cai-Bao Hu^2,18, Guo-Rong Lyu¹⁹, Xin-Ru Qiu^1,2, Li-Qun Jia²⁰, Xiao-Man Wang²⁰, Xiao-Ling Ren^1,2, Jiu-Ye Guo^1,2, Yue-Qiao Gao^1,2, Jian-Jun Li^1,2, Ying Liu^1,2, Wei Fu^1,2, Yan Wang²¹, Zu-Lin Lu^1,2, Hua-Wei Wang⁸, Li-Li Shang²²

¹Department of Neonatology and NICU, Beijing Chaoyang District Maternal and Child Healthcare Hospital, ²The Neonatal Lung Ultrasound Training Base, Chinese College of Critical Ultrasound, ³Emergency Department, Cattinara University Hospital, ⁴Division of Pediatric Radiology, Department of Radiology, Medical University Graz, ⁵Faculty of Medicine, University of Novi Sad, Radiology Department, Institute for Children and Adolescents Health Care of Vojvodina, ⁶Pediatric Intensive Care Unit, Pediatric Service Hospital Joan XXIII Tarragona, University Rovira i Virgil, ⁷Division of Neonatal-Perinatal Medicine, Cohen Children's Medical Center, ⁸Department of Neonatology, Children's Hospital of Soochow University, ⁹Department of Neonatology, Udine University Hospital, ¹⁰Center for Newborn Care, Guangzhou Women and Children's Medical Center, ¹¹Division of Neonatology, Children's Hospital of Philadelphia, ¹²Department of Radiology, Children's Hospital of Philadelphia, ¹³Division of Neonatology and NICU, Cook County Children's Hospital, University of Illinois, ¹⁴Division of Neonatology, Department of Pediatrics, Taipei Medical University, ¹⁵Department of Pediatrics and Community Health Sciences, University of Calgary, ¹⁶Department of Pediatrics, The Third Affiliated Hospital of Xinxiang Medical University, ¹⁷Department of Ultrasound, GE Healthcare, ¹⁸Intensive Care Unit, Zhejiang Hospital, ¹⁹Collaborative Innovation Center for Maternal and Infant Health Service Application Technology, Quanzhou Medical College, ²⁰Department of Ultrasound, Beijing Children's Hospital Affiliated with Capital Medical University, ²¹Department of Neonatology and NICU, Tai'an City Central Hospital of Shandong Province, ²²Department of Intensive Care Unit, The Second Affiliated Hospital of Heilongjiang University of Chinese Medicine

Lung ultrasound is a noninvasive and valuable tool for bedside evaluation of neonatal lung diseases. However, a relative lack of reference standards, protocols and guidelines may limit its application. Here, we aim to develop a standardized neonatal lung ultrasound diagnostic protocol to be used in clinical decision-making.

Biology

Laser-Capture Microdissection RNA-Sequencing for Spatial and Temporal Tissue-Specific Gene Expression Analysis in Plants

Lim Chee Liew^1,2, Yan Wang^1,2, Marta Peirats-Llobet¹, Oliver Berkowitz^1,2,3, James Whelan^1,2,3, Mathew G. Lewsey^1,3

¹Department of Animal, Plant and Soil Science, AgriBio Building, La Trobe University, ²Australian Research Council Centre of Excellence in Plant Energy Biology, La Trobe University, ³Australian Research Council Research Hub for Medicinal Agriculture, AgriBio Building, La Trobe University

Presented here is a protocol for laser-capture microdissection (LCM) of plant tissues. LCM is a microscopic technique for isolating areas of tissue in a contamination-free manner. The procedure includes tissue fixation, paraffin embedding, sectioning, LCM and RNA extraction. RNA is used in the downstream tissue-specific, temporally resolved analysis of transcriptomes.