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NO 984 Hospital of PLA

9 ARTICLES PUBLISHED IN JoVE

Medicine

Modeling Myotonic Dystrophy 1 in C2C12 Myoblast Cells

Rui Liang^*1, Wei Dong^*1,3, Xiaopeng Shen¹, Xiaoping Peng^1,3, Angie G. Aceves^1,2, Yu Liu¹

¹Department of Biology and Biochemistry, University of Houston, ²Department of Economics, University of Houston, ³Department of Cardiology, The First Affiliated Hospital of Nanchang University

In this protocol, we present the procedures in establishing myotonic dystrophy 1 myoblast models, including optimized C2C12 cell maintenance, gene transfection/transduction, and myocyte differentiation.

Biology

Pancreatic Islet Embedding for Paraffin Sections

Yahui Kong¹, Pantea Ebrahimpour^1,2, Yu Liu³, Chaoxing Yang¹, Laura C. Alonso¹

¹Diabetes Center of Excellence, UMass Medical School, ²Department of Medicine, Saint Vincent Hospital, ³Department of Pathology, Morphology Core, UMass Medical School

Ex vivo pancreatic islet studies are important for diabetes research. Existing techniques to study cultured islets in their native 3-dimensional architecture are time consuming, inefficient, and infrequently used. This work describes a new, simple, and efficient method for generating high-quality paraffin sections of whole cultured islets.

Neuroscience

Sub-acute Cerebral Microhemorrhages Induced by Lipopolysaccharide Injection in Rats

Dandan Li^*1,2, Hóngyi Zhào^*2,3, Wei Wei², Nan Liu², Yonghua Dr. Huang²

¹Department of Neurology, Second Hospital of Shanxi Medical University, ²Department of Neurology, PLA Army General Hospital, ³Department of Neurology, NO 261 Hospital of PLA

We present a protocol to induce and detect CMHs caused by LPS injection in Sprague-Dawley rats, which may be utilized in future research investigations on the pathogenesis of CMHs.

Medicine

Traditional Trail Making Test Modified into Brand-new Assessment Tools: Digital and Walking Trail Making Test

Wei Wei^*1, Hóngyi Zhào^*1,2, Yu Liu², Yonghua Huang¹

¹Department of Neurology, The Seventh Medical Center of PLA Army General Hospital, ²Department of Neurology, NO 984 Hospital of PLA

Here, we present a protocol to show how to perform two types of cognitive assessment tools derived from the paper-pencil version of the Trail Making Test.

Medicine

Digital Handwriting Analysis of Characters in Chinese Patients with Mild Cognitive Impairment

Hóngyi Zhào^*1,2, Yanhai Zhang^*3, Cuiqiao Xia¹, Yu Liu², Zhiyi Li², Yonghua Huang¹

¹Department of Neurology, Chinese PLA General Hospital, ²Department of Neurology, NO 984 Hospital of PLA, ³Department of Cadre Ward, Xijing 986 Hospital, Fourth Military Medical University

This study proposed a digital handwriting analysis of characters in individuals with mild cognitive impairment to find more information than is revealed by traditional pencil–paper handwriting analysis.

Cancer Research

Characterization and Functional Prediction of Bacteria in Ovarian Tissues

Lanbo Zhao^*1, Weichu Zhao^*2, Qi Wang^*1, Dongxin Liang¹, Yu Liu³, Guoxing Fu⁴, Lu Han¹, Yiran Wang¹, Chao Sun¹, Qing Wang¹, Qing Song¹, Qiling Li¹, Qinrui Lu¹

¹Department of Obstetrics and Gynecology, First Affiliated Hospital of Xi’an Jiaotong University, ²School of Social Sciences, University of Manchester, ³Department of Pathology, First Affiliated Hospital of Xi’an Jiaotong University, ⁴Omega Bioservices Inc

Immunohistochemistry staining and 16S ribosomal RNA gene (16S rRNA gene) sequencing were performed in order to discover and distinguish bacteria in cancerous and noncancerous ovarian tissues in situ. The compositional and functional differences of the bacteria were predicted by using BugBase and Phylogenetic Investigation of Communities by Reconstruction of Unobserved States (PICRUSt).

Behavior

Physical Activity Measurement in Children Accepting Table Tennis Training

Xin Zhang^*1, Cuiqiao Xia^*2, Xiuli Zhao³, Yu Liu³, Hóngyi Zhào^2,3, Yonghua Huang²

¹Department of Sport, Inner Mongolia Medical University, ²Department of Neurology, The Seventh Medical Center of PLA General Hospital, ³Department of Neurology, NO 984 Hospital of PLA

This study proposes an accelerometer-based method to objectively measure physical activity (PA) and leisure time physical activity (LTPA) in Chinese children accepting table tennis training in clubs.

Medicine

Using Motion Capture Technology in the Instrumented Timed Up and Go Test to Detect the Risk of Falling in Aged Adults

Xiaoping Cai^*1,2, Hongyi Zhao^*1,3, Xinjun Shan⁴, Yonghua Huang¹, Fangyuan Wei⁵

¹Department of Neurology, The Seventh Medical Center of Chinese PLA General Hospital, ²7th Department of Health Cadre, The Second Medical Center of Chinese PLA General Hospital, ³Department of Neurology, NO 984 Hospital of PLA, ⁴Department of Emergency, General Hospital of Baoding First Central Hospital, ⁵Department of Hand and Foot Surgery, Beijing University of Chinese Medicine Third Affiliated Hospital

The instrumented Timed Up and Go (iTUG) test is gaining increasing attention in body sway and gait analysis with the development of new technologies. We present a protocol to analyze the subcomponents of the iTUG with motion capture.

Medicine

Association Between Sleep Quality and Cognitive Symptoms in Patients with Major Depressive Disorder

Rui Zhao^*1, Zhiqiang Zhang^*1, Hong Li², Dongsheng Lv¹, Ping Wang³, Hongyi Zhao³

¹Sleep Medicine Center of Inner Mongolia Mental Health Center (The Third Hospital of Inner Mongolia Autonomous Region, Brain Hospital of Inner Mongolia Autonomous Region), ²Department of Neurosurgery, NO 984 Hospital of PLA, ³Department of Neurology, NO 984 Hospital of PLA

Here, we present a protocol that investigates the relationship between cognitive symptoms and sleep quality in patients with major depressive disorder. The THINC-integrated tool and polysomnography were used to evaluate cognitive symptoms and sleep quality.