This method can help scientists maximize productive use of pancreatic islets to assess multiple outcomes on each sample in its native tissue architecture. The main advantage of this new embedding method is that islets are evenly distributed over a
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Ex vivo pancreatic islet studies are important for diabetes research. Existing techniques to study cultured islets in their native 3-dimensional architecture are time consuming, inefficient, and infrequently used. This work describes a new, simple, and efficient method for generating high-quality paraffin sections of whole cultured islets.